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Activation of Toll-like receptor 7/8 encoded by the X chromosome alters sperm motility and provides a novel simple technology for sexing sperm

https://hiroshima.repo.nii.ac.jp/records/2007929
https://hiroshima.repo.nii.ac.jp/records/2007929
c3e1b980-26a4-4100-9959-3f8e6938c3f9
名前 / ファイル ライセンス アクション
pbio_3000398.pdf pbio_3000398.pdf (3.1 MB)
Item type デフォルトアイテムタイプ_(フル)(1)
公開日 2023-03-18
タイトル
タイトル Activation of Toll-like receptor 7/8 encoded by the X chromosome alters sperm motility and provides a novel simple technology for sexing sperm
言語 en
作成者 Umehara, Takashi

× Umehara, Takashi

en Umehara, Takashi

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Tsujita, Natsumi

× Tsujita, Natsumi

en Tsujita, Natsumi

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Shimada, Masayuki

× Shimada, Masayuki

en Shimada, Masayuki

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
権利情報 © 2019 Umehara et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
内容記述
内容記述 In most mammals, the male to female sex ratio of offspring is about 50% because half of the sperm contain either the Y chromosome or X chromosome. In mice, the Y chromosome encodes fewer than 700 genes, whereas the X chromosome encodes over 3,000 genes. Although overall gene expression is lower in sperm than in somatic cells, transcription is activated selectively in round spermatids. By regulating the expression of specific genes, we hypothesized that the X chromosome might exert functional differences in sperm that are usually masked during fertilization. In this study, we found that Toll-like receptors 7/8 (TLR7/8) coding the X chromosome were expressed by approximately 50% of the round spermatids in testis and in approximately 50% of the epididymal sperm. Especially, TLR7 was localized to the tail, and TLR8 was localized to the midpiece. Ligand activation of TLR7/8 selectively suppressed the mobility of the X chromosome–bearing sperm (X-sperm) but not the Y-sperm without altering sperm viability or acrosome formation. The difference in sperm motility allowed for the separation of Y-sperm from X-sperm. Following in vitro fertilization using the ligand-selected high-mobility sperm, 90% of the embryos were XY male. Likewise, 83% of the pups obtained following embryo transfer were XY males. Conversely, the TLR7/8-activated, slow mobility sperm produced embryos and pups that were 81% XX females. Therefore, the functional differences between Y-sperm and X-sperm motility were revealed and related to different gene expression patterns, specifically TLR7/8 on X-sperm.
言語 en
内容記述
内容記述タイプ Other
内容記述 This work was supported in part by Livestock Promotional Funds of Japan Racing Association (JRA) and by Hiroshima Cryopreservation Service Co. (to MS).
出版者
出版者 Public Library of Science
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
関連情報
識別子タイプ DOI
関連識別子 10.1371/journal.pbio.3000398
関連情報
識別子タイプ DOI
関連識別子 https://doi.org/10.1371/journal.pbio.3000398
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 1545-7885
開始ページ
開始ページ e3000398
書誌情報 PLoS Biology
PLoS Biology

巻 17, 号 8, p. e3000398, 発行日 2019-08-13
旧ID 48136
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