ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

  1. 学術雑誌論文等

Fungicidal effect of three new synthetic cationic peptides against Candida albicans

https://hiroshima.repo.nii.ac.jp/records/2007910
https://hiroshima.repo.nii.ac.jp/records/2007910
427e5002-358c-47aa-b6c6-7b24f07b05a3
名前 / ファイル ライセンス アクション
OralDis_10_221.pdf OralDis_10_221.pdf (1.3 MB)
Item type デフォルトアイテムタイプ_(フル)(1)
公開日 2023-03-18
タイトル
タイトル Fungicidal effect of three new synthetic cationic peptides against Candida albicans
言語 en
作成者 Nikawa, Hiroki

× Nikawa, Hiroki

en Nikawa, Hiroki

Search repository
Fukashima, H.

× Fukashima, H.

en Fukashima, H.

Search repository
Makihira, Seicho

× Makihira, Seicho

en Makihira, Seicho

Search repository
Hamada, Taizo

× Hamada, Taizo

en Hamada, Taizo

Search repository
Samaranayake, L.P.

× Samaranayake, L.P.

en Samaranayake, L.P.

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
権利情報 Copyright (c) 2004 Blackwell Munksgaard.
権利情報
権利情報 The difinitive version is available at www.blackwell-synergy.com
主題
主題Scheme Other
主題 cationic peptide
主題
主題Scheme Other
主題 antifungal activity
主題
主題Scheme Other
主題 Candida albicans
主題
主題Scheme NDC
主題 490
内容記述
内容記述 OBJECTIVE: Peptide antibiotics are considered a new class of antifungal agents. Of these, an α-helical, cationic peptide termed Dhvar 4, a relative of salivary histatin has been shown to be an antifungal of relatively high potency. Similarly, lactoferricin B (LFB) and a derivative thereof, LFB(17-30), disrupts the fungal cell membrane and acts against Candida albicans. As Dhvar 4 and LFB(17-30), exhibit almost identical amino acid sequences at their C-terminal, we hypothesized that laboratory synthesis of peptides with an α-helical structure and having similar amphipathic properties could lead to products with candidacidal activity. Hence, three such peptides - JH8194, JH8195 and JH 8944, were synthesized and their antifungal properties compared with recognized antifungals LFB, LFB(17-30), human lactoferricin (LFH), Histatin-5 and Dhvar 4, against two isolates of C. albicans. MATERIALS AND METHODS: The antifungal agents were synthesized and their secondary structures evaluated according to a previously described protocol of Situ and Bobek (2000) Antimicrob Agents Chemother 44: 1485-1493. The C. albicans strains were oral isolates from a human immunodeficiency virus-infected (isolate A2) and a healthy (A6) individual. A standard concentration of yeasts was exposed to a range of dilutions of the agents for a specific duration and the cell death (viability) in terms of the resultant colony forming units ml-1 was quantified. RESULTS: Dhvar 4, showed the most α-helical propensity, and was the least fungicidal while LFB and LFB(17-30) showed the highest antifungal potential, and demonstrated total kill of A6, and A2 at 5 and 10 μM concentrations, respectively whilst LFH killed both isolates at a 10 μM concentration. Of the three new synthetic peptides, JH 8194 was the most potent (total kill of A6/A2 strains at 1.25/2.5 μM), followed by JH 8195 (total kill of A6/A2 strains at 5/10 μM while JH 8944 was the least potent as a 25 μM concentration was required to kill either strain of Candida. On further analyses of the relationship between pl value of the peptides and their anticandicidal activity, a significant positive correlation was noted. In order to rule out a cytotoxic effect of the new synthetic peptides we compared the fungicidal and hemolytic activities under similar incubation conditions using freshly isolated erythrocytes and all three peptides exhibited no detectable hemolysis upto an concentration of 100 μM in contrast to the polyene antifungal amphotericin B that elicited significant initiation of hemolysis at a concentration of 5.0 μM. CONCLUSION: Our data suggest that laboratory synthesis of agents with an α-helical structure and having amphipathic properties similar to known, natural antifungal agents may be a promising avenue to generate products with improved antifungal activity.
言語 en
出版者
出版者 Blackwell
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
出版タイプ
出版タイプ AO
出版タイプResource http://purl.org/coar/version/c_b1a7d7d4d402bcce
関連情報
識別子タイプ DOI
関連識別子 10.1111/j.1601-0825.2004.01010.x
関連情報
識別子タイプ PMID
関連識別子 15196144
関連情報
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 http://dx.doi.org/10.1111/j.1601-0825.2004.01010.x
収録物識別子
収録物識別子タイプ NCID
収録物識別子 AA11044105
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 1354-523X
開始ページ
開始ページ 221
書誌情報 Oral Diseases
Oral Diseases

巻 10, 号 4, p. 221-228, 発行日 2004-07
旧ID 19888
戻る
0
views
See details
Views

Versions

Ver.1 2025-02-21 04:06:58.271116
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR 2.0
  • OAI-PMH JPCOAR 1.0
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3