| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
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|
タイトル |
Genes encoded within 8q24 on the amplicon of a large extrachromosomal element are selectively repressed during the terminal differentiation of HL-60 cells |
|
言語 |
en |
| 作成者 |
Hirano, Tetsuo
Ike, Fumio
Murata, Takehide
Obata, Yuichi
Utiyama, Hiroyasu
Yokoyama, Kazunari K.
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2007 Elsevier B.V. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
gene amplification |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
genetic instability |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
double minute chromosome |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
large extrachromosomal element |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
terminal differentiation |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
apoptosis |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
460 |
| 内容記述 |
|
|
内容記述 |
Human acute myeloblastic leukemia HL-60 cells become resistant to differentiation during longterm cultivation. After 150 passages, double minute chromosomes (dmins) found in early-passaged cells are replaced by large extrachromosomal elements (LEEs). In a DNA library derived from a purified fraction of LEEs, 12.6% (23/183) of clones were assigned to 8q24 and 9.2% (17/183) were assigned to 14q11 in the human genome. Fluorescence in situ hybridization (FISH) revealed a small aberrant chromosome, which had not been found in early-passaged cells, in addition to the purified LEEs. We determined that each LEE consisted of six discontinuous segments in a region that extended for 4.4 Mb over the 8q24 locus. Five genes, namely, Myc (a proto-oncogene), NSMCE2 (for a SUMO ligase), CCDC26 (for a retinoic acid-dependent modulator of myeloid differentiation), TRIB1 (for a regulator of MAPK kinase) and LOC389637 (for a protein of unknown function), were encoded by the amplicon. Breaks in the chromosomal DNA within the amplicon were found in the NSMCE2 and CCDC26 genes. The discontinuous structure of the amplicon unit of the LEEs was identical with that of dmins in HL60 early-passaged cells. The difference between them seemed, predominantly, to be the number (10 to 15 copies per LEE versus2 or 3 copies per dmin) of constituent units. Expression of the Myc, NSMCE2, CCDC26 and LOC389637 and TRIB1genes was constitutive in all lines of HL-60 cells and that of the first four genes was repressed during the terminal differentiation of earlypassaged HL-60 cells. We also detected abnormal transcripts of CCDC26. Our results suggest that these genes were selected during the development of amplicons. They might be amplified and, sometimes, truncated to contribute to the maintenance of HL-60 cells in an undifferentiated state. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier B.V. |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
10.1016/j.mrfmmm.2007.12.008 |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.mrfmmm.2007.12.008 |
| 収録物識別子 |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11032004 |
| 収録物識別子 |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0027-5107 |
| 開始ページ |
|
|
開始ページ |
97 |
| 書誌情報 |
Mutation Research : Fundamental and Molecular Mechanisms of Mutagenesis
Mutation Research : Fundamental and Molecular Mechanisms of Mutagenesis
巻 640,
号 1-2,
p. 97-106,
発行日 2008
|
| 旧ID |
20819 |
MutationRes_Hirano.pdf (2.1 MB)
