ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

  1. 学術雑誌論文等

Genes encoded within 8q24 on the amplicon of a large extrachromosomal element are selectively repressed during the terminal differentiation of HL-60 cells

https://hiroshima.repo.nii.ac.jp/records/2007750
https://hiroshima.repo.nii.ac.jp/records/2007750
afbafde7-c872-48ef-911d-5a71da57b6cc
名前 / ファイル ライセンス アクション
MutationRes_Hirano.pdf MutationRes_Hirano.pdf (2.1 MB)
Item type デフォルトアイテムタイプ_(フル)(1)
公開日 2023-03-18
タイトル
タイトル Genes encoded within 8q24 on the amplicon of a large extrachromosomal element are selectively repressed during the terminal differentiation of HL-60 cells
言語 en
作成者 Hirano, Tetsuo

× Hirano, Tetsuo

en Hirano, Tetsuo

Search repository
Ike, Fumio

× Ike, Fumio

en Ike, Fumio

Search repository
Murata, Takehide

× Murata, Takehide

en Murata, Takehide

Search repository
Obata, Yuichi

× Obata, Yuichi

en Obata, Yuichi

Search repository
Utiyama, Hiroyasu

× Utiyama, Hiroyasu

en Utiyama, Hiroyasu

Search repository
Yokoyama, Kazunari K.

× Yokoyama, Kazunari K.

en Yokoyama, Kazunari K.

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
権利情報 Copyright (c) 2007 Elsevier B.V.
主題
主題Scheme Other
主題 gene amplification
主題
主題Scheme Other
主題 genetic instability
主題
主題Scheme Other
主題 double minute chromosome
主題
主題Scheme Other
主題 large extrachromosomal element
主題
主題Scheme Other
主題 terminal differentiation
主題
主題Scheme Other
主題 apoptosis
主題
主題Scheme NDC
主題 460
内容記述
内容記述 Human acute myeloblastic leukemia HL-60 cells become resistant to differentiation during long­term cultivation. After 150 passages, double minute chromosomes (dmins) found in early­-passaged cells are replaced by large extrachromosomal elements (LEEs). In a DNA library derived from a purified fraction of LEEs, 12.6% (23/183) of clones were assigned to 8q24 and 9.2% (17/183) were assigned to 14q11 in the human genome. Fluorescence in situ hybridization (FISH) revealed a small aberrant chromosome, which had not been found in early­-passaged cells, in addition to the purified LEEs. We determined that each LEE consisted of six discontinuous segments in a region that extended for 4.4 Mb over the 8q24 locus. Five genes, namely, Myc (a proto­-oncogene), NSMCE2 (for a SUMO ligase), CCDC26 (for a retinoic acid­-dependent modulator of myeloid differentiation), TRIB1 (for a regulator of MAPK kinase) and LOC389637 (for a protein of unknown function), were encoded by the amplicon. Breaks in the chromosomal DNA within the amplicon were found in the NSMCE2 and CCDC26 genes. The discontinuous structure of the amplicon unit of the LEEs was identical with that of dmins in HL­60 early­-passaged cells. The difference between them seemed, predominantly, to be the number (10 to 15 copies per LEE versus2 or 3 copies per dmin) of constituent units. Expression of the Myc, NSMCE2, CCDC26 and LOC389637 and TRIB1genes was constitutive in all lines of HL­-60 cells and that of the first four genes was repressed during the terminal differentiation of early­passaged HL­-60 cells. We also detected abnormal transcripts of CCDC26. Our results suggest that these genes were selected during the development of amplicons. They might be amplified and, sometimes, truncated to contribute to the maintenance of HL­-60 cells in an undifferentiated state.
言語 en
出版者
出版者 Elsevier B.V.
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
出版タイプ
出版タイプ AO
出版タイプResource http://purl.org/coar/version/c_b1a7d7d4d402bcce
関連情報
識別子タイプ DOI
関連識別子 10.1016/j.mrfmmm.2007.12.008
関連情報
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 http://dx.doi.org/10.1016/j.mrfmmm.2007.12.008
収録物識別子
収録物識別子タイプ NCID
収録物識別子 AA11032004
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0027-5107
開始ページ
開始ページ 97
書誌情報 Mutation Research : Fundamental and Molecular Mechanisms of Mutagenesis
Mutation Research : Fundamental and Molecular Mechanisms of Mutagenesis

巻 640, 号 1-2, p. 97-106, 発行日 2008
旧ID 20819
戻る
0
views
See details
Views

Versions

Ver.1 2025-02-21 04:01:52.480220
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR 2.0
  • OAI-PMH JPCOAR 1.0
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3