| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Direct binding of RalA to PKC eta and its crucial role in morphological change during keratinocyte differentiation |
|
言語 |
en |
| 作成者 |
Shirai, Yasuhito
Morioka, Shoko
Sakuma, Megumi
Yoshino, Ken-ichi
Otsuji, Chihiro
Sakai, Norio
Kashiwagi, Kaori
Chida, Kazuhiro
Shirakawa, Ryutaro
Horiuchi, Hisanori
Nishigori, Chikako
Ueyama, Takehiko
Saito, Naoaki
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| アクセス権 |
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|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2011 Shirai et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution-Noncommercial-Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
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内容記述 |
During differentiation, keratinocytes undergo a dramatic shape change from small and round to large and flat, in addition to production of proteins necessary for the formation of epidermis. It has been shown that protein kinase C (PKC) η is crucial for keratinocyte differentiation. However, its role in this process has yet to be fully elucidated. Here, we show that catalytic activity is not necessary for enlarged and flattened morphology of human keratinocytes induced by overexpression of PKCη, although it is important for gene expression of the marker proteins. In addition, we identify the small G protein RalA as a binding partner of PKCη, which binds to the C1 domain, an indispensable region for the morphological change. The binding led activation of RalA and actin depolymerization associated with keratinocyte differentiation. siRNA techniques proved that RalA is involved in not only the keratinocyte differentiation induced by PKCη overexpression but also normal keratinocyte differentiation induced by calcium and cholesterol sulfate. These results provide a new insight into the molecular mechanism of cytoskeletal regulation leading to drastic change of cell shape. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
The American Society for Cell Biology |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1091/mbc.E10-09-0754 |
| 関連情報 |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1091/mbc.E10-09-0754 |
| 収録物識別子 |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12024500 |
| 開始ページ |
|
|
開始ページ |
1340 |
| 書誌情報 |
Molecular biology of the cell
Molecular biology of the cell
巻 22,
号 8,
p. 1340-1352,
発行日 2011-02-23
|
| 旧ID |
32028 |
MolBiolCell_22_1340.pdf (2.9 MB)
