| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Molecular Characterization of the Schizosaccharomyces pombe nbs1+ Gene Involved in DNA Repair and Telomere Maintenance |
|
言語 |
en |
| 作成者 |
Ueno, Masaru
Nakazaki, Tomofumi
Akamatsu, Yufuko
Watanabe, Kikuo
Tomita, Kazunori
Lindsay, Howard D.
Shinagawa, Hideo
Iwasaki, Hiroshi
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2003 American Society for Microbiology |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
MRN |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
nbs1 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
methyl methanesulfonate |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
genetic disease |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Nijmegen breakage syndrome |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
420 |
| 内容記述 |
|
|
内容記述 |
The human MRN complex is a multisubunit nuclease that is composed of Mre11, Rad50, and Nbs1 and is involved in homologous recombination and DNA damage checkpoints. Mutations of the MRN genes cause genetic disorders such as Nijmegen breakage syndrome. Here we identified a Schizosaccharomyces pombe nbs1+ homologue by screening for mutants with mutations that caused methyl methanesulfonate (MMS) sensitivity and were synthetically lethal with the rad2△ mutation. Nbs1 physically interacts with the C-terminal half of Rad32, the Schizosaccharomyces pombe Mre11 homologue, in a yeast two-hybrid assay. nbs1 mutants showed sensitivities to γ-rays, UV, MMS, and hydroxyurea and displayed telomere shortening similar to the characteristics of rad32 and rad50 mutants. nbs1, rad32, and rad50 mutant cells were elongated and exhibited abnormal nuclear morphology. These findings indicate that S. pombe Nbs1 forms a complex with Rad32-Rad50 and is required for homologous recombination repair, telomere length regulation, and the maintenance of chromatin structure. Amino acid sequence features and some characteristics of the DNA repair function suggest that the S. pombe Rad32-Rad50-Nbs1 complex has functional similarity to the corresponding MRN complexes of higher eukaryotes. Therefore, S. pombe Nbs1 will provide an additional model system for studying the molecular function of the MRN complex associated with genetic diseases. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
American Society for Microbiology |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1128/MCB.23.18.6553-6563.2003 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0270-7306 |
| 開始ページ |
|
|
開始ページ |
6553 |
| 書誌情報 |
Molecular and cellular biology
Molecular and cellular biology
巻 23,
p. 6553-6563,
発行日 2003-09
|
| 旧ID |
14206 |
Mol-cell-biol_23_6553-6563_2003.pdf (1.8 MB)
