| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
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|
タイトル |
Competition between the Rad50 Complex and the Ku Heterodimer Reveals a Role for Exo1 in Processing Double-Strand Breaks but Not Telomeres |
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言語 |
en |
| 作成者 |
Tomita, Kazunori
Matsuura, Akira
Caspari, Thomas
Carr, Antony M.
Akamatsu, Yufuko
Iwasaki, Hiroshi
Mizuno, Ken-ichi
Ohta, Kunihiro
Uritani, Masahiro
Ushimaru, Takashi
Yoshinaga, Koichi
Ueno, Masaru
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| アクセス権 |
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アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
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|
権利情報 |
Copyright (c) American Society for Microbiology |
| 主題 |
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主題Scheme |
NDC |
|
主題 |
460 |
| 内容記述 |
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|
内容記述 |
The Mre11-Rad50-Nbs1(Xrs2) complex and the Ku70-Ku80 heterodimer are thought to compete with each other for binding to DNA ends. To investigate the mechanism underlying this competition, we analyzed both DNA damage sensitivity and telomere overhangs in Schizosaccharomyces pombe rad50-d, rad50-d pku70-d, rad50-d exo1-d, and pku70-d rad50-d exo1-d cells. We found that rad50 exo1 double mutants are more methyl methanesulfonate (MMS) sensitive than the respective single mutants. The MMS sensitivity of rad50-d cells was suppressed by concomitant deletion of pku70+. However, the MMS sensitivity of the rad50 exo1 double mutant was not suppressed by the deletion of pku70+. The G-rich overhang at telomere ends in taz1-d cells disappeared upon deletion of rad50+, but the overhang reappeared following concomitant deletion of pku70+. Our data suggest that the Rad50 complex can process DSB ends and telomere ends in the presence of the Ku heterodimer. However, the Ku heterodimer inhibits processing of DSB ends and telomere ends by alternative nucleases in the absence of the Rad50-Rad32 protein complex. While we have identified Exo1 as the alternative nuclease targeting DNA break sites, the identity of the nuclease acting on the telomere ends remains elusive. |
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言語 |
en |
| 出版者 |
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|
出版者 |
American Society for Microbiology |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.1128/MCB.23.15.5186-5197.2003 |
| 関連情報 |
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|
識別子タイプ |
PMID |
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関連識別子 |
12861005 |
| 関連情報 |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1128/MCB.23.15.5186-5197.2003 |
| 収録物識別子 |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0270-7306 |
| 開始ページ |
|
|
開始ページ |
5186 |
| 書誌情報 |
Molecular and cellular biology
Molecular and cellular biology
巻 23,
p. 5186-5197,
発行日 2003-08
|
| 旧ID |
14204 |
Mol-cell-biol_23_5186-5197_2003.pdf (6.8 MB)
