| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Fission Yeast Pot1 and RecQ Helicase Are Required for Efficient Chromosome Segregation |
|
言語 |
en |
| 作成者 |
Takahashi, Katsunori
Imano, Ryota
Kibe, Tatsuya
Seimiya, Hiroyuki
Muramatsu, Yukiko
Kawabata, Naoki
Tanaka, Genki
Matsumoto, Yoshitake
Hiromoto, Taisuke
Koizumi, Yuka
Nakazawa, Norihiko
Yanagida, Mitsuhiro
Yukawa, Masashi
Tsuchiya, Eiko
Ueno, Masaru
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) American Society for Microbiology, Mol. Cell. Biol. February 2011 vol. 31 no. 3 495-506, doi: 10.1128/MCB.00613-10 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Telomere |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Pot1 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
RecQ helicase |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Anti-microtubule drug |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
460 |
| 内容記述 |
|
|
内容記述 |
Pot1 is a single-stranded telomere-binding protein that is conserved from fission yeast to mammals. Deletion of Schizosaccharomyces pombe pot1+ causes immediate telomere loss. S. pombe Rqh1 is a homolog of the human RecQ helicase WRN, which plays essential roles in the maintenance of genomic stability. Here, we demonstrate that a pot1Δ rqh1-hd (helicase-dead) double mutant maintains telomeres that are dependent on Rad51-mediated homologous recombination. Interestingly, the pot1Δ rqh1-hd double mutant displays a “cut" (cell untimely torn) phenotype and is sensitive to the antimicrotubule drug thiabendazole (TBZ). Moreover, the chromosome ends of the double mutant do not enter the pulsed-field electrophoresis gel. These results suggest that the entangled chromosome ends in the pot1Δ rqh1-hd double mutant inhibit chromosome segregation, signifying that Pot1 and Rqh1 are required for efficient chromosome segregation. We also found that POT1 knockdown, WRN-deficient human cells are sensitive to the antimicrotubule drug vinblastine, implying that some of the functions of S. pombe Pot1 and Rqh1 may be conserved in their respective human counterparts POT1 and WRN. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
American Society for Microbiology |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1128/MCB.00613-10 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1128/MCB.00613-10 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0270-7306 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10620925 |
| 開始ページ |
|
|
開始ページ |
495 |
| 書誌情報 |
Molecular and Cellular Biology
Molecular and Cellular Biology
巻 31,
号 3,
p. 495-506,
発行日 2011-02
|
| 旧ID |
33044 |
MolCellBiol_31_495.pdf (1.3 MB)
