| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
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タイトル |
Fission yeast MOZART1/Mzt1 is an essential γ-tubulin complex component required for complex recruitment to the microtubule organizing center, but not its assembly |
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言語 |
en |
| 作成者 |
Masuda, Hirohisa
Mori, Risa
Yukawa, Masashi
Toda, Takashi
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| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
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権利情報 |
© 2013 Masuda et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). |
| 内容記述 |
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内容記述 |
γ-Tubulin plays a universal role in microtubule nucleation from microtubule organizing centers (MTOCs) such as the animal centrosome and fungal spindle pole body (SPB). γ-Tubulin functions as a multiprotein complex called the γ-tubulin complex (γ-TuC), consisting of GCP1–6 (GCP1 is γ-tubulin). In fungi and flies, it has been shown that GCP1–3 are core components, as they are indispensable for γ-TuC complex assembly and cell division, whereas the other three GCPs are not. Recently a novel conserved component, MOZART1, was identified in humans and plants, but its precise functions remain to be determined. In this paper, we characterize the fission yeast homologue Mzt1, showing that it is essential for cell viability. Mzt1 is present in approximately equal stoichiometry with Alp4/GCP2 and localizes to all the MTOCs, including the SPB and interphase and equatorial MTOCs. Temperature-sensitive mzt1 mutants display varying degrees of compromised microtubule organization, exhibiting multiple defects during both interphase and mitosis. Mzt1 is required for γ-TuC recruitment, but not sufficient to localize to the SPB, which depends on γ-TuC integrity. Intriguingly, the core γ-TuC assembles in the absence of Mzt1. Mzt1 therefore plays a unique role within the γ-TuC components in attachment of this complex to the major MTOC site. |
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言語 |
en |
| 内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
This research was supported by Cancer Research UK (T.T.). |
| 出版者 |
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出版者 |
The American Society for Cell Biology |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
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識別子タイプ |
DOI |
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関連識別子 |
10.1091/mbc.e13-05-0235 |
| 関連情報 |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1091/mbc.e13-05-0235 |
| 関連情報 |
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識別子タイプ |
PMID |
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関連識別子 |
23885124 |
| 収録物識別子 |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1059-1524 |
| 収録物識別子 |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1939-4586 |
| 開始ページ |
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開始ページ |
2894 |
| 書誌情報 |
Molecular Biology of the Cell
Molecular Biology of the Cell
巻 24,
号 18,
p. 2894-2906,
発行日 2013-09-15
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| 旧ID |
48779 |
MBoC_24_2894.pdf (3.0 MB)
