| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Vanadocytes, Cells hold the Key to Resolving the Highly Selective Accumulation and Reduction of Vanadium in Ascidians |
|
言語 |
en |
| 作成者 |
Michibata, Hitoshi
Uyama, Taro
Ueki, Tatsuya
Kanamori, Kan
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2002 Wiley-Liss, Inc. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Ascidian |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Vanadium |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Accumulation |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Reduction |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
pH |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Vacular H+-ATPase |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Monoclonal antibody |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
460 |
| 内容記述 |
|
|
内容記述 |
Since Henze discovered vanadium in the blood (or coelomic) cells of an ascidian in 1911, this unusual phenomenon has attracted the interest of many investigators. The highest concentration of vanadium (350 mM) in the blood cells of Ascidia gemmata, which belongs to the suborder Phlebobranchia, is 107 times higher than that in sea water. Of the approximately ten types of blood cells, a combination of cell fractionation and neutron-activation analysis revealed that the signet ring cells were the true vanadocytes. In the vanadocytes, 97.60f the vanadium is in the +3 oxidation state (III). The extremely low pH of 1.9 found in vanadocytes suggests that protons, concentrated by an H+-ATPase, might be linked to the accumulation of vanadium energetically. The antigen recognized by a monoclonal antibody, S4D5, prepared to identify vanadocytes, was determined to be 6-PGDH in the pentose phosphate pathway. NADPH produced in the pentose phosphate pathway in vanadocytes is thought to participate in the reduction of vanadium(V) to vanadium(IV). During embryogenesis, a vanadocyte-specific antigen first appears in the body wall at the same time as significant accumulations of vanadium become apparent. Three different vanadium-associated proteins (VAPs) were extracted from the blood cells of vanadium-rich ascidians. These are 12.5, 15, and 16 kDa in size and are associated with vanadium in an approximate ratio of 1:16. The cDNA encoding the 12.5 and 15 kDa VAPs was isolated and the proteins encoded were found to be novel. Further biochemical and biophysical characterization of the VAPs is in progress. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Wiley-Liss, Inc. |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1002/jemt.10042 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
11921344 |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1002/jemt.10042 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1059-910X |
| 開始ページ |
|
|
開始ページ |
421 |
| 書誌情報 |
Microsopy research and technique
Microsopy research and technique
巻 56,
p. 421-434,
発行日 2002
|
| 旧ID |
22 |
MicroscopResTech_56_421-434_2002.pdf (764.1 KB)
