| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Branched-chain amino acids-induced cardiac protection against ischemia/reperfusion injury |
|
言語 |
en |
| 作成者 |
Satomi, Shiho
Morio, Atsushi
Miyoshi, Hirotsugu
Nakamura, Ryuji
Tsutsumi, Rie
Sakaue, Hiroshi
Yasuda, Toshimichi
Saeki, Noboru
Tsutsumi, Yasuo M.
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| 権利情報 |
|
|
権利情報 |
This is not the published version. Please cite only the published version. この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Amino acid |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Ischemia |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Reperfusion |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
mTOR |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Mitochondria |
| 内容記述 |
|
|
内容記述 |
Aims: Amino acids, especially branched chain amino acids (BCAAs), have important regulatory roles in protein synthesis. Recently studies revealed that BCAAs protect against ischemia/reperfusion (I/R) injury. We studied the signaling pathway and mitochondrial function affecting a cardiac preconditioning of BCAAs. Main methods: An in vivo model of I/R injury was tested in control, mTOR+/+, and mTOR+/−. Mice were randomly assigned to receive BCAAs, rapamycin, or BCAAs + rapamycin. Furthermore, isolated cardiomyocytes were subjected to simulated ischemia and cell death was quantified. Biochemical and mitochondrial swelling assays were also performed. Key findings: Mice treated with BCAAs had a significant reduction in infarct size as a percentage of the area at risk compared to controls (34.1 ± 3.9% vs. 44.7 ± 2.6%, P = 0.001), whereas mice treated with the mTOR inhibitor rapamycin were not protected by BCAA administration (42.2 ± 6.5%, vs. control, P = 0.015). This protection was not detected in our hetero knockout mice of mTOR. Western blot analysis revealed no change in AKT signaling whereas activation of mTOR was identified. Furthermore, BCAAs prevented swelling which was reversed by the addition of rapamycin. In myocytes undergoing simulated I/R, BCAA treatment significantly preserved cell viability (71.7 ± 2.7% vs. 34.5 ± 1.6%, respectively, p < 0.0001), whereas rapamycin prevented this BCAA-induced cardioprotective effect (43.5 ± 3.4% vs. BCAA, p < 0.0001). Significance: BCAA treatment exhibits a protective effect in myocardial I/R injury and that mTOR plays an important role in this preconditioning effect. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
This work was supported by JSPS KAKENHI, Japan [grant number 19K09353]. |
| 出版者 |
|
|
出版者 |
Elsevier |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.lfs.2020.117368 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
32001270 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.lfs.2020.117368 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0024-3205 |
| 開始ページ |
|
|
開始ページ |
117368 |
| 書誌情報 |
Life Sciences
Life Sciences
巻 245,
p. 117368,
発行日 2020-03-15
|
| 旧ID |
50462 |
| 備考 |
Post-print version/PDF may be used in an institutional repository after an embargo period of 12 months. |
LifeSci_245_117368.pdf (873.7 KB)
