| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Studies on Morphology and Cytochemistry in Blood Cells of Ayu Plecoglossus altivelis altivelis |
|
言語 |
en |
| 作成者 |
Nakada, Kojin
Fujisawa, Kuniyasu
Horiuchi, Hiroyuki
Furusawa, Shuichi
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2014 The Japanese Society of Veterinary Science |
| 権利情報 |
|
|
権利情報 |
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License <http://creativecommons.org/licenses/by-nc-nd/3.0/>. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
ayu |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
characterization |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
classification |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Percoll |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
peripheral blood |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
640 |
| 内容記述 |
|
|
内容記述 |
Peripheral blood cells from ayu, Plecoglossus altivelis altivelis, were separated using a density gradient. Blood cells were then smeared using Shandon Cytospin and subjected to cytochemical staining. Blood cells were categorized based on morphological and cytochemical characteristics, and the density fractionation range and nucleus area/cell area ratio were observed. Lymphocytes are distinguished from neutrophils by their basophilic cytoplasm and Golgi-like field. The features of chromatin in thrombocytes are different from those of lymphocytes or neutrophils, but some small neutrophils have similar chromatin. Therefore, it is necessary to perform peroxidase staining to distinguish small neutrophils from thrombocytes. Basophils have large basophilic granules in cytoplasm. Based on density fractionation of blood cells, thrombocytes in the low-density area were separated from other blood cells. Identification of peripheral blood cells from ayu was possible with these staining methods. Monocytes/macrophages from spleen are specifically positive for esterase staining by α-naphthyl butyrate. As a result, thrombocytes, lymphocytes, neutrophils, basophils and monocytes/macrophages were identified in smears from peripheral blood or spleen tissue. In this paper, we confirmed that the peripheral blood corpuscles of ayu are able to be identified using the present staining methods. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
公益社団法人日本獣医学会 |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1292/jvms.13-0584 |
| 関連情報 |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
https://www.jstage.jst.go.jp/article/jvms/76/5/76_13-0584/_article/ |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1347-7439 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0916-7250 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10796138 |
| 開始ページ |
|
|
開始ページ |
693 |
| 書誌情報 |
Journal of Veterinary Medical Science
Journal of Veterinary Medical Science
巻 76,
号 5,
p. 693-704,
発行日 2014-01-28
|
| 旧ID |
45392 |
JVetMedSci_76_693.pdf (2.0 MB)
