| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2024-12-16 |
| タイトル |
|
|
タイトル |
Periodontal tissue regeneration with cementogenesis after application of brain-derived neurotrophic factor in 3-wall inflamed intra-bony defect |
|
言語 |
en |
| 作成者 |
Kiyota, Mari
Iwata, Tomoyuki
Hasegawa, Naohiko
Sasaki, Shinya
Taniguchi, Yuri
Hamamoto, Yuta
Matsuda, Shinji
Ouhara, Kazuhisa
Takeda, Katsuhiro
Fujita, Tsuyoshi
Kurihara, Hidemi
Kawaguchi, Hiroyuki
Mizuno, Noriyoshi
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is the peer reviewed version of the following article: Journal of Periodontal Research: Volume 59, Issue 3, Pages 530-541, which has been published in final form at https://doi.org/10.1111/jre.13244. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is not the published version. Please cite only the published version. |
| 権利情報 |
|
|
言語 |
ja |
|
権利情報 |
この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。 |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
3-wall intra-bony defect |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
atelocollagen |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
brain-derived neurotrophic factor (BDNF) |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
periodontal tissue regeneration |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
regenerative therapy |
| 内容記述 |
|
|
内容記述 |
Objective The purpose of this study is to investigate regenerative process by immunohistochemical analysis and evaluate periodontal tissue regeneration following a topical application of BDNF to inflamed 3-wall intra-bony defects. Background Brain-derived neurotrophic factor (BDNF) plays a role in the survival and differentiation of central and peripheral neurons. BDNF can regulate the functions of non-neural cells, osteoblasts, periodontal ligament cells, endothelial cells, as well as neural cells. Our previous study showed that a topical application of BDNF enhances periodontal tissue regeneration in experimental periodontal defects of dog and that BDNF stimulates the expression of bone (cementum)-related proteins and proliferation of human periodontal ligament cells. Methods Six weeks after extraction of mandibular first and third premolars, 3-wall intra-bony defects were created in mandibular second and fourth premolars of beagle dogs. Impression material was placed in all of the artificial defects to induce inflammation. Two weeks after the first operation, BDNF (25 and 50 μg/mL) immersed into atelocollagen sponge was applied to the defects. As a control, only atelocollagen sponge immersed in saline was applied. Two and four weeks after the BDNF application, morphometric analysis was performed. Localizations of osteopontin (OPN) and proliferating cell nuclear antigen (PCNA)-positive cells were evaluated by immunohistochemical analysis. Results Two weeks after application of BDNF, periodontal tissue was partially regenerated. Immunohistochemical analyses revealed that cells on the denuded root surface were positive with OPN and PCNA. PCNA-positive cells were also detected in the soft connective tissue of regenerating periodontal tissue. Four weeks after application of BDNF, the periodontal defects were regenerated with cementum, periodontal ligament, and alveolar bone. Along the root surface, abundant OPN-positive cells were observed. Morphometric analyses revealed that percentage of new cementum length and percentage of new bone area of experimental groups were higher than control group and dose-dependently increased. Conclusion These findings suggest that BDNF could induce cementum regeneration in early regenerative phase by stimulating proliferation of periodontal ligament cells and differentiation into periodontal tissue cells, resulting in enhancement of periodontal tissue regeneration in inflamed 3-wall intra-bony defects. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
John Wiley & Sons Ltd. |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1111/jre.13244 |
| 開始ページ |
|
|
開始ページ |
530 |
| 書誌情報 |
en : Journal of Periodontal Research
巻 59,
号 3,
p. 530-541,
発行日 2024-03-19
|
| 旧ID |
55864 |
JPeriodontalRes_59_530.pdf (3.8 MB)
