| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2024-12-16 |
| タイトル |
|
|
タイトル |
Periostin regulates integrin expression in gingival epithelial cells |
|
言語 |
en |
| 作成者 |
Reika Hirata
Iwata, Tomoyuki
Fujita, Tsuyoshi
Nagahara, Takayoshi
Matsuda, Shinji
Sasaki, Shinya
Taniguchi, Yuri
Hamamoto, Yuta
Ouhara, Kazuhisa
Kudo, Yasusei
Kurihara, Hidemi
Mizuno, Noriyoshi
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
© 2023. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is not the published version. Please cite only the published version. |
| 権利情報 |
|
|
言語 |
ja |
|
権利情報 |
この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。 |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Gingival epithelial cells |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Mesenchymal stem cells |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Human periodontal ligament cells |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Periostin |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Integrin beta |
| 内容記述 |
|
|
内容記述 |
Objective Human gingival epithelial cells (HGECs) function as a mechanical barrier against invasion by pathogenic organisms through epithelial cell–cell junction complexes, which are complex components of integrin. Integrins play an important role in the protective functions of HGECs. Human periodontal ligament (HPL) cells regulate periodontal homeostasis. However, periodontitis results in the loss of HPL cells. Therefore, as replenishment, HPL cells or mesenchymal stem cells (MSCs) can be transplanted. Herein, HPL cells and MSCs were used to elucidate the regulatory mechanisms of HGECs, assuming periodontal tissue homeostasis. Methods Human gingival fibroblasts (HGFs), HGECs, HPL cells, and MSCs were cultured, and the conditioned medium was collected. With or without silencing periostin mRNA, HGECs were cultured under normal conditions or in a conditioned medium. Integrin and periostin mRNA expression was determined using real-time polymerase chain reaction. Integrin protein expression was analyzed using flow cytometry, and periostin protein expression was determined via western blotting. Results The conditioned medium affected integrin expression in HGECs. Higher expression of periostin was observed in MSCs and HPL cells than in HGFs. The conditioned medium that contained periostin protein regulated integrin expression in HGECs. After silencing periostin in MSCs and HPL cells, periostin protein was not detected in the conditioned medium, and integrin expression in HGECs remained unaffected. Conclusions Integrins in HGECs are regulated by periostin secreted from HPL cells and MSCs. This result suggests that periostin maintains gingival cell adhesion and regulates bacterial invasion/infection. Therefore, the functional regulation of periostin-secreting cells is important in preventing periodontitis. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.job.2023.11.009 |
| 開始ページ |
|
|
開始ページ |
170 |
| 書誌情報 |
en : Journal of Oral Biosciences
巻 66,
号 1,
p. 170-178,
発行日 2023-12-03
|
| 旧ID |
55863 |
JOB_66_170.pdf (1.3 MB)
