WEKO3
アイテム
Effects of S-glutathionylation on the passive force–length relationship in skeletal muscle fibres of rats and humans
https://hiroshima.repo.nii.ac.jp/records/2007414
https://hiroshima.repo.nii.ac.jp/records/20074145ad1e2a6-600d-44c6-b7ac-2b7fc61604e1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
JMRCM_41_239.pdf (1.2 MB)
|
| Item type | デフォルトアイテムタイプ_（フル）(1) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2023-03-18 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Effects of S-glutathionylation on the passive force–length relationship in skeletal muscle fibres of rats and humans | |||||||||||
| 言語 | en | |||||||||||
| 作成者 |
Watanabe, Daiki
× Watanabe, Daiki
× Lamboley, Cedric R.
× Lamb, Graham D.
|
|||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | open access | |||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||
| 権利情報 | ||||||||||||
| 権利情報 | This is not the published version. Please cite only the published version. この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。 | |||||||||||
| 主題 | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Oxidative stress | |||||||||||
| 主題 | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Muscle elasticity | |||||||||||
| 主題 | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Skinned fbre | |||||||||||
| 主題 | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Titin | |||||||||||
| 主題 | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Passive force | |||||||||||
| 内容記述 | ||||||||||||
| 内容記述 | This study investigated the efect of S-glutathionylation on passive force in skeletal muscle fbres, to determine whether activity-related redox reactions could modulate the passive force properties of muscle. Mechanically-skinned fbres were freshly obtained from human and rat muscle, setting sarcomere length (SL) by laser difraction. Larger stretches were required to produce passive force in human fbres compared to rat fbres, but there were no fbre-type diferences in either species. When fbres were exposed to glutathione disulfde (GSSG; 20 mM, 15 min) whilst stretched (at a SL where passive force reached~20% of maximal Ca2+-activated force, denoted as SL20 % max), passive force was subsequently decreased at all SLs in both type I and type II fbres of rat and human (e.g., passive force at SL20 % max decreased by 12 to 25%). This decrease was fully reversed by subsequent reducing treatment with dithiothreitol (DTT; 10 mM for 10 min). If freshly skinned fbres were initially treated with DTT, there was an increase in passive force in type II fbres (by 10±3% and 9±2% in rat and human fbres, respectively), but not in type I fbres. These results indicate that (i) S-glutathionylation, presumably in titin, causes a decrease in passive force in skeletal muscle fbres, but the reduction is relatively smaller than that reported in cardiac muscle, (ii) in rested muscle in vivo, there appears to be some level of reversible oxidative modifcation, probably involving S-glutathionylation of titin, in type II fbres, but not in type I fbres. | |||||||||||
| 言語 | en | |||||||||||
| 内容記述 | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | We thank the National Health and Medical Research Council of Australia for financial support (Grant No. 1085331). | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | Springer | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| 出版タイプ | ||||||||||||
| 出版タイプ | AO | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_b1a7d7d4d402bcce | |||||||||||
| 関連情報 | ||||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | 10.1007/s10974-019-09563-5 | |||||||||||
| 関連情報 | ||||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | https://doi.org/10.1007/s10974-019-09563-5 | |||||||||||
| 収録物識別子 | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 0142-4319 | |||||||||||
| 収録物識別子 | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 1573-2657 | |||||||||||
| 開始ページ | ||||||||||||
| 開始ページ | 239 | |||||||||||
| 書誌情報 |
Journal of Muscle Research and Cell Motility Journal of Muscle Research and Cell Motility 巻 41, p. 239-250, 発行日 2019-11-02 |
|||||||||||
| 旧ID | 51508 | |||||||||||
