| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
The Expression and Roles of Semaphorin Type 3C in Granulosa Cells during The Luteinization Process |
|
言語 |
en |
| 作成者 |
Okabe, Ayami
Hiramatsu, Rui
Umehara, Takashi
Fujita, Youko
Shimada, Masayuki
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
© 2014 Japan Society of Ova Research |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
luteinization |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Migration |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Granulosa cells |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Semaphorin |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Ovulation |
| 内容記述 |
|
|
内容記述 |
Morphological changes are observed during the luteinization process of granulosa cells, however mechanisms and the roles are remained unclear. In the present study, we demonstrated that Sema3C is expressed in granulosa cells and then secreted to extracellular matrix accumulated within cumulus cell and granulosa cell layers. The expression is dependent on EGF-like factors (amphiregulin and neuregulin 1) and EGF receptor dependent manner. The knockdown of Sema3c not only significantly decreased the surface area of the granulosa cells, but also significantly suppressed the cell migration of granulosa cells cultured with EGF like factors. The phosphorylation of focal adhesion kinase (FAK) was dramatically suppressed by transfection with Sema3c siRNA, with an increasing level of phosphorylated ROCK, indicating that Sema3C regulated actin remodeling in a FAK signaling pathway-dependent manner. Moreover, the expression levels of genes involved in progesterone production, Star, Cyp11a1 were significantly decreased in Sema3c siRNA-transfected granulosa cells as compared with control siRNA transfection. From these results, we conclude that Sema3C is secreted and then accumulated within granulosa cell layers and cumulus cell layers. The FAK pathway is activated by Sema3C and induces cell migration and enlarges the area of granulosa cells. These morphological changes are required for luteinization (production of progesterone) of granulosa cells. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
This work was supported, in part, by a grant-in-Aid for Scientific Research No. 24688028, and No. 21688019 (M. S.) from the Japan Society for the Promotion of Science (JSPS). |
| 出版者 |
|
|
出版者 |
Japan Society of Ova Research |
| 出版者 |
|
|
出版者 |
日本卵子学会 |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1274/jmor.31.31 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1274/jmor.31.31 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1347-5878 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1341-7738 |
| 開始ページ |
|
|
開始ページ |
31 |
| 書誌情報 |
Journal of Mammalian Ova Research
Journal of Mammalian Ova Research
巻 31,
号 1,
p. 31-39,
発行日 2014
|
| 旧ID |
48663 |
JMammOvaRes_31_31.pdf (1.0 MB)
