| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Growth and differentiation of colony-forming human hepatocytes in vitro |
|
言語 |
en |
| 作成者 |
Yamasaki, Chihiro
Tateno, Chise
Aratani, Akio
Ohnishi, Chimoto
Katayama, Shigeru
Kohashi, Toshihiko
Hino, Hiroshi
Marusawa, Hiroyuki
Asahara, Toshimasa
Yoshizato, Katsutoshi
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| アクセス権 |
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|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2006 Elsevier Ltd. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Progenitor cells |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Human hepatocytes |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Spheroids |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Matrigel |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Hepatocyte propagation |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Albumin |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
HNF4 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Cytochrome P450 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Cytokeratins |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
3-dimensional culture |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
460 |
| 内容記述 |
|
|
内容記述 |
Background/Aims: Parenchymal hepatocytes (PHs) of rat contain colony-forming parenchymal hepatocytes (CF-PHs) as a small fraction. We aimed to demonstrate the presence of CF-PHs in humans and characterize them with respect to growth and differentiation potential. Methods: Human PHs were co-cultured with Swiss 3T3 cells in the medium containing human serum, EGF, nicontinamide, and ascorbic acid 2- phosphate. To examine differentiation potential hepatocytes were cultured on gels of Matrigel Matrix. Results: Few PHs formed colonies, the colony-forming efficiency being as low as 0.01-0.09%.0 The CF-PHs could be subcultured up to 7 passages. They showed a liver epithelial cell-like morphology, and immunocytochemically positive for albumin (ALB), cytokeratin (CK) 7, 8, 18, and 19 in a pre- and early phase-confluence, whereas they showed a typical differentiated hepatocyte-like morphology, and positive for α1-antitrypsin, but negative for CK7 and 19 in condensed regions at confluence. The CF-PHs at late confluence expressed mRNAs of ALB, HNF4, and isoforms of cytochrome P450 at low levels. However, when cultured on Matrigel, these cells expressed them at high levels comparable to those of original PHs. Conclusions: We concluded that the human liver contains highly replicative hepatic progenitor-like cells as a minute population that retain a normal differentiation potential. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.jhep.2005.10.028 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
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関連識別子 |
16469405 |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.jhep.2005.10.028 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0168-8278 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10459105 |
| 開始ページ |
|
|
開始ページ |
749 |
| 書誌情報 |
Journal of Hepatology
Journal of Hepatology
巻 44,
号 4,
p. 749-757,
発行日 2006-04
|
| 旧ID |
17093 |
JourHepatol_44_749.pdf (3.7 MB)
