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Slow molecular dynamics close to crystal surfaces during crystallization of a protein lysozyme studied by fluorescence correlation spectroscopy
https://hiroshima.repo.nii.ac.jp/records/2007281
https://hiroshima.repo.nii.ac.jp/records/200728124aafe64-a171-436b-9531-d4d3aadf0da4
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
JChemPhys_133_095103.pdf (669.7 KB)
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| Item type | デフォルトアイテムタイプ_（フル）(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2023-03-18 | |||||||
| タイトル | ||||||||
| タイトル | Slow molecular dynamics close to crystal surfaces during crystallization of a protein lysozyme studied by fluorescence correlation spectroscopy | |||||||
| 言語 | en | |||||||
| 作成者 |
Tanaka, Shinpei
× Tanaka, Shinpei
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| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 権利情報 | ||||||||
| 権利情報 | Copyright (c) 2010 American Institute of Physics. | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | crystallisation | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | fluorescence | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | optical correlation | |||||||
| 主題 | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | proteins | |||||||
| 主題 | ||||||||
| 主題Scheme | NDC | |||||||
| 主題 | 430 | |||||||
| 内容記述 | ||||||||
| 内容記述 | Fluorescence correlation spectroscopy FCS was applied to the crystallization processes of egg-white lysozyme. Utilizing FCS's high spatial resolution of about the laser wavelength used, the molecular dynamics close to crystal surfaces was investigated for both tetragonal single crystals and needlelike spherulites. When the FCS measurement was done at the point closer than 1 m to the surface of a tetragonal single crystal, the relaxation time became several times longer than that in bulk solution, but the fluorescence intensity thus concentration was similar to that observed in bulk solution. On the other hand, the peculiar slow dynamics a few orders of magnitude slower than that in bulk solution of concentrated liquid states of the lysozyme molecules was observed in needlelike spherulites. We suggested that these observations could be explained by the formation of softly connected aggregates accumulating around the needlelike crystals, which could cause the instability of the crystal growth and thus the formation of spherulites. These aggregates gradually disappeared as the crystallization further proceeded. After the disappearance of the aggregates, the spherulites started to mature. | |||||||
| 言語 | en | |||||||
| 出版者 | ||||||||
| 出版者 | The American Institute of Physics | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | journal article | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 関連情報 | ||||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | 10.1063/1.3478224 | |||||||
| 関連情報 | ||||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | http://dx.doi.org/10.1063/1.3478224 | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0021-9606 | |||||||
| 収録物識別子 | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA00694991 | |||||||
| 開始ページ | ||||||||
| 開始ページ | 095103-1 | |||||||
| 書誌情報 |
Journal of Chemical Physics Journal of Chemical Physics 巻 133, 号 9, p. 095103-1-095103-9, 発行日 2010 |
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| 旧ID | 30777 | |||||||
