| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
An unconventional interaction between Dis1/TOG and Mal3/EB1 in fission yeast promotes the fidelity of chromosome segregation |
|
言語 |
en |
| 作成者 |
Matsuo, Yuzy
Maurer, Sebastian P.
Yukawa, Masashi
Zakian, Silva
Singleton, Martin R.
Surrey, Thomas
Toda, Takashi
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
© 2016. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Crystallography |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
EB1 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Microtubule polymerase |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
TIRF microscopy |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
XMAP215 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
TOG |
| 内容記述 |
|
|
内容記述 |
Dynamic microtubule plus-ends interact with various intracellular target regions such as the cell cortex and the kinetochore. Two conserved families of microtubule plus-end-tracking proteins, the XMAP215, ch-TOG or CKAP5 family and the end-binding 1 (EB1, also known as MAPRE1) family, play pivotal roles in regulating microtubule dynamics. Here, we study the functional interplay between fission yeast Dis1, a member of the XMAP215/TOG family, and Mal3, an EB1 protein. Using an in vitro microscopy assay, we find that purified Dis1 autonomously tracks growing microtubule ends and is a bona fide microtubule polymerase. Mal3 recruits additional Dis1 to microtubule ends, explaining the synergistic enhancement of microtubule dynamicity by these proteins. A non-canonical binding motif in Dis1 mediates the interaction with Mal3. X-ray crystallography shows that this new motif interacts in an unconventional configuration with the conserved hydrophobic cavity formed within the Mal3 C-terminal region that typically interacts with the canonical SXIP motif. Selectively perturbing the Mal3–Dis1 interaction in living cells demonstrates that it is important for accurate chromosome segregation. Whereas, in some metazoans, the interaction between EB1 and the XMAP215/TOG family members requires an additional binding partner, fission yeast relies on a direct interaction, indicating evolutionary plasticity of this critical interaction module. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
This work was supported by Cancer Research UK and the Francis Crick Institute which receives its core funding from Cancer Research UK (FC001155, FC001163, FC001184), the UK Medical Research Council (FC001155, FC001163, FC001184), and the Wellcome Trust (FC001155, FC001163, FC001184), the Japan Society for the Promotion of Science (JSPS) [KAKENHI Scientific Research (A) (16H02503 to T.T.), a Challenging Exploratory Research grant (16K14672 to T.T.), Scientific Research (C) (16K07694 to M.Y.)], the Naito Foundation (T.T.) and a Marie Curie fellowship from the European Commission (PIEF-GA-2009-253043 to S.P.M.). |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Supplementary information available online at http://jcs.biologists.org/lookup/doi/10.1242/jcs.197533.supplemental |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1242/jcs.197533 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1242/jcs.197533 |
| 関連情報 |
|
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|
識別子タイプ |
PMID |
|
|
関連識別子 |
27872152 |
| 収録物識別子 |
|
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0021-9533 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1477-9137 |
| 開始ページ |
|
|
開始ページ |
4592 |
| 書誌情報 |
Journal of Cell Science
Journal of Cell Science
巻 129,
号 24,
p. 4592-4606,
発行日 2016
|
| 旧ID |
48775 |
JCellSci_129_4592.pdf (2.9 MB)
