| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
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タイトル |
Selected Mutations in a Mesophilic Cytochrome c Confer the Stability of a Thermophilic Counterpart |
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言語 |
en |
| 作成者 |
Hasegawa, Jun
Uchiyama, Susumu
Tanimoto, Yuko
Mizutani, Masayuki
Kobayashi, Yuji
Sambongi, Yoshihiro
Igarashi, Yasuo
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| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
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|
権利情報 |
This research was originally published in the Journal of Biological Chemistry. Jun Hasegawa, Susumu Uchiyama, Yuko Tanimoto, Masayuki Mizutani, Yuji Kobayashi, Yoshihiro Sambongi and Yasuo Igarashi. Selected Mutations in a Mesophilic Cytochrome c Confer the Stability of a Thermophilic Counterpart. J. Biol. Chem. 2000; 275(48):37824-37828. © the American Society for Biochemistry and Molecular Biology. |
| 内容記述 |
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内容記述 |
Mesophilic cytochrome c551 of Pseudomonas aeruginosa (PA c551) became as stable as its thermophilic counterpart, Hydrogenobacter thermophilus cytochrome c552 (HT c552), through only five amino acid substitutions. The five residues, distributed in three spatially separated regions, were selected and mutated with reference to the corresponding residues in HT c552 through careful structure comparison. Thermodynamic analysis indicated that the stability of the quintuple mutant of PA c551 could be partly attained through an enthalpic factor. The solution structure of the mutant showed that, as in HT c552, there were tighter side chain packings in the mutated regions. Furthermore, the mutant had an increased total accessible surface area, resulting in great negative hydration free energy. Our results provide a novel example of protein stabilization in that limited amino acid substitutions can confer the overall stability of a natural highly thermophilic protein upon a mesophilic molecule. |
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言語 |
en |
| 内容記述 |
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内容記述タイプ |
Other |
|
内容記述 |
This work was supported by a grant from the Japanese Ministry of Education, Science and Culture. |
| 出版者 |
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出版者 |
The American Society for Biochemistry and Molecular Biology, Inc. |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版タイプ |
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|
出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
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識別子タイプ |
DOI |
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関連識別子 |
10.1074/jbc.M005861200 |
| 関連情報 |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.1074/jbc.M005861200 |
| 収録物識別子 |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0021-9258 |
| 収録物識別子 |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1083-351X |
| 開始ページ |
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開始ページ |
37824 |
| 書誌情報 |
Journal of Biological Chemistry
Journal of Biological Chemistry
巻 275,
号 48,
p. 37824-37828,
発行日 2000-12-01
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| 旧ID |
48608 |
J. Biol. Chem_275_37824.pdf (283.8 KB)
