| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Monolithic silica capillary column as a new tool for extraction of methamphetamine in urine |
|
言語 |
en |
| タイトル |
|
|
タイトル |
モノリス型カラムを用いた尿中メタンフェタミンの抽出 |
|
言語 |
ja |
| 作成者 |
Namera, Akira
Nakamoto, Akihiro
Nishida, Manami
Yashiki, Mikio
Kuramoto, Takako
Takei, Yoshiyuki
Furuno, Masahiro
Minakuchi, Hiroyoshi
Nakanishi, Kazuki
Kimura, Kojiro
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
(c) 2004 Japanese Association of Forensic Toxicology. |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
Methamphetamine |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Monolithic silica capillary column |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Solid-phase extraction |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Spot test |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Simon's reaction |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
|
|
内容記述 |
We have established a simple screening method for methamphetamine (MA) in urine using a monolithic silica capillary column for extraction and using the Simon's reagent for colorimetric determination. Urine was mixed with borate buffer solution (50 mM, pH 10.2) and the mixture was passed through the column connected with the needle of a gas-tight syringe. MA in urine was adsorbed to the column, then eluted with 10 µl of ethyl acetate, and directly spotted on a thin-layer chromatographic plate by dividing the eluate into several aliquots. To separate MA from urine impurities and detect it clearly, a 2-µl volume of a solvent mixture (chloroform : methanol = 95 : 5, v/v, saturated with 28-0x1.fa060bfff8bbp+0mmonium hydroxide) was applied to the spot for concentrically development of the analyte. After drying the solvent, 0.5% sodium nitroprusside solution containing 10% sodium carbonate was sprayed on the plate. The plate was put in a bottle saturated with acetaldehyde, and the ring was changed purple (Simon's reaction) when MA existed in urine. The macroscopic detection limit of MA in urine by this method was 0.5 µg/ml. To confirm efficiency of this method, the eluate from the column was analyzed by GC/MS using penta-deuterated MA as internal standard. By analysis of 30 urine samples, the results obtained by the present spot test agreed well with those by GC/MS. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Japanese Association of Forensic Toxicology |
| 出版者 |
|
|
出版者 |
日本法中毒学会 |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
http://wwwsoc.nii.ac.jp/jaft/ |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0915-9606 |
| 開始ページ |
|
|
開始ページ |
200 |
| 書誌情報 |
Japanese journal of forensic toxicology
Japanese journal of forensic toxicology
巻 22,
号 3,
p. 200-204,
発行日 2004
|
| 旧ID |
30119 |
