| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
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|
タイトル |
Gingival epithelial cells heterozygous for Toll-like receptor 4 polymorphisms Asp299Gly and Thr399Ile are hypo-responsive to Porphyromonas gingivalis |
|
言語 |
en |
| 作成者 |
Kinane, Denis F.
Shiba, Hideki
Stathopoulou, Panagiota G.
Zhao, Hongli
Lappin, David F.
Singh, Amar
Eskan, Mehmet A.
Beckers, Simone
Weigel, Sabine
Alpert, Brian
Knudsen, Thomas B.
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| アクセス権 |
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|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2006 Nature Publishing Group. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Toll-like receptors |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
periodontitis |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
epithelial cells |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
tissue culture |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
disease susceptibility |
| 内容記述 |
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|
内容記述 |
The Toll-like receptor (TLR4) is the major sensor for bacterial lipopolysaccharide and its two common co-segregating polymorphisms, Asp299Gly and Thr399Ile, which occur at a frequency of between 6 and 10%, have been associated with infectious diseases, LPS hypo-responsiveness and cardiovascular disease. Porphyromonas gingivalis is a Gram negative bacterium implicated in chronic periodontitis and is a known TLR4 and TLR2 agonist. We obtained two gingival epithelial cell primary cultures from subjects heterozygous for the TLR4 polymorphism Asp299Gly and compared response characteristics with similar cells from patients (four) with the wild type TLR4 genes. Cytokine responses and transcriptome profiles of gingival epithelial cell primary culture cells to TNFα challenge were similar for all primary epithelial cell cultures. P. gingivalis challenge however, gave markedly different responses for Asp299Gly heterozygous and wild-type epithelial cell cultures. The epithelial cells heterozygous for the TLR4 polymorphism Asp299Gly were functionally hypo-responsive, evidenced by differences in BD-2 mRNA expression, mRNA response profile by microarray analysis and by pro-inflammatory and chemokine cytokines at the protein and mRNA level. These findings emphasize variance in human epithelial cell TLRs, linked with Asp299Gly carriage, which results in a hypo-responsive epithelial cell phenotype less susceptible to Gram negative diseases and associated systemic conditions. |
|
言語 |
en |
| 出版者 |
|
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出版者 |
Nature Publishing Group |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.1038/sj.gene.6364282 |
| 関連情報 |
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|
識別子タイプ |
PMID |
|
|
関連識別子 |
16437123 |
| 関連情報 |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1038/sj.gene.6364282 |
| 収録物識別子 |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1466-4879 |
| 収録物識別子 |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11474931 |
| 開始ページ |
|
|
開始ページ |
190 |
| 書誌情報 |
Genes and Immunity
Genes and Immunity
巻 7,
号 3,
p. 190-200,
発行日 2006-04
|
| 旧ID |
17075 |
GenesImm_7_190.pdf (434.0 KB)
