| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
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|
タイトル |
Difference in NaCl tolerance of membrane-bound 5′-nucleotidases purified from deep-sea and brackish water Shewanella species |
|
言語 |
en |
| 作成者 |
Kuribayashi, Taka-aki
Fujii, Sotaro
Masanari, Misa
Yamanaka, Masaru
Wakai, Satoshi
Sambongi, Yoshihiro
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| アクセス権 |
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|
アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
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|
権利情報 |
© Springer Japan 2016 |
| 権利情報 |
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|
権利情報 |
This is a post-peer-review, pre-copyedit version of an article published in Extremophiles. The final authenticated version is available online at: https://doi.org/10.1007/s00792-016-0909-8 |
| 権利情報 |
|
|
権利情報 |
This is not the published version. Please cite only the published version. この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。 |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
Deep-sea |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
Growth environment |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
5′-Nucleotidase |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
NaCl tolerance |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
Shewanella |
| 内容記述 |
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|
内容記述 |
Shewanella species are widely distributed in sea, brackish, and fresh water areas, growing psychrophilically or mesophilically, and piezophilically or piezo-sensitively. Here, membrane-bound 5′-nucleotidases (NTases) from deep-sea Shewanella violacea and brackish water Shewanella amazonensis were examined from the aspect of NaCl tolerance in order to gain an insight into protein stability against salt. Both NTases were single polypeptides with molecular masses of ~59 kDa, as determined on mass spectroscopy. They similarly required 10 mM MgCl2 for their activities, and they exhibited the same pH dependency and substrate specificity for 5′-nucleotides. However, S. violacea 5′-nucleotidase (SVNTase) was active enough in the presence of 2.5 M NaCl, whereas S. amazonensis 5′-nucleotidase (SANTase) exhibited significantly reduced activity with the same concentration of the salt. Although SVNTase and SANTase exhibited high sequence identity (69.7%), differences in the ratio of acidic to basic amino acid residues and the number of potential salt bridges maybe being responsible for the difference in the protein stability against salt. 5′-Nucleotidases from these Shewanella species will provide useful information regarding NaCl tolerance, which may be fundamental for understanding bacterial adaptation to growth environments. |
|
言語 |
en |
| 内容記述 |
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|
内容記述タイプ |
Other |
|
内容記述 |
This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan [No. 26240045], a grant from the Japan Society for the Promotion of Science [No. 25-1446], and The Salt Science Research Foundation [No. 1655]. |
| 出版者 |
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出版者 |
Springer Verlag |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
10.1007/s00792-016-0909-8 |
| 関連情報 |
|
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|
識別子タイプ |
PMID |
|
|
関連識別子 |
28050644 |
| 関連情報 |
|
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1007/s00792-016-0909-8 |
| 収録物識別子 |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1431-0651 |
| 収録物識別子 |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1433-4909 |
| 開始ページ |
|
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開始ページ |
357 |
| 書誌情報 |
Extremophiles
Extremophiles
巻 21,
号 2,
p. 357-368,
発行日 2017-01-03
|
| 旧ID |
48645 |
Extremophiles_21_357.pdf (981.2 KB)
