| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2025-01-17 |
| タイトル |
|
|
タイトル |
Human cranial bone-derived mesenchymal stem cells cultured under simulated microgravity can improve cerebral infarction in rats |
|
言語 |
en |
| 作成者 |
Kuwabara, Masashi
Mitsuhara, Takafumi
Teranishi, Masataka
Okazaki, Takahito
Takeda, Masaaki
Ishii, Daizo
Kondo, Hiroshi
Shimizu, Kiyoharu
Hosogai, Masahiro
Hara, Takeshi
Maeda, Yuyo
Kurose, Tomoyuki
Kawahara, Yumi
Yuge, Louis
Horie, Nobutaka
|
| アクセス権 |
|
|
アクセス権 |
embargoed access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_f1cf |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
© 2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is not the published version. Please cite only the published version. |
| 権利情報 |
|
|
言語 |
ja |
|
権利情報 |
この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。 |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Mesenchymal stem cells |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Simulated microgravity |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Cerebral infarction |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Human cranial bone |
| 内容記述 |
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|
内容記述 |
The efficacy of transplanting human cranial bone-derived mesenchymal stem cells (hcMSCs) cultured under simulated microgravity (sMG) conditions has been previously reported; however, their effect on cerebral infarction remains unknown. Here, we examined the efficacy of transplanting hcMSCs cultured in an sMG environment into rat models of cerebral infarction. For evaluating neurological function, hcMSCs cultured in either a normal gravity (1G) or an sMG environment were transplanted in rats 1 day after inducing cerebral infarction. The expression of endogenous neurotrophic, axonal, neuronal, synaptogenic, angiogenic, and apoptosis-related factors in infarcted rat brain tissue was examined using real-time polymerase chain reaction and western blotting 35 days after stroke induction. The RNAs of hcMSCs cultured under 1G or sMG environments were sequenced. The results showed that neurological function was significantly improved after transplantation of hcMSCs from the sMG group compared with that from the 1G group. mRNA expressions of nerve growth factor, fibroblast growth factor 2, and synaptophysin were significantly higher in the sMG group than in the 1G group, whereas sortilin 1 expression was significantly lower. RNA sequencing analysis revealed that genes related to cell proliferation, angiogenesis, neurotrophy, neural and synaptic organization, and inhibition of cell differentiation were significantly upregulated in the sMG group. In contrast, genes promoting microtubule and extracellular matrix formation and cell adhesion, signaling, and differentiation were downregulated. These results demonstrate that hcMSCs cultured in the sMG environment may be a useful source of stem cells for the recovery of neurological function after cerebral infarction. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.expneurol.2024.114947 |
| 助成情報 |
|
|
|
助成機関名 |
日本学術振興会 |
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言語 |
ja |
|
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研究課題番号URI |
https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-20K09348/ |
|
|
研究課題番号 |
20K09348 |
|
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研究課題名 |
模擬微小重力環境で培養したヒト頭蓋骨由来間葉系幹細胞の脳梗塞ラットへの移植効果 |
|
|
言語 |
ja |
| 助成情報 |
|
|
|
助成機関名 |
Japan Society for the Promotion of Science |
|
|
言語 |
en |
|
|
研究課題名 |
Effects of transplantation of human cranial bone-derived mesenchymal stem cells cultured in a simulated microgravity on rat cerebral infarction model |
| 開始ページ |
|
|
開始ページ |
114947 |
| 書誌情報 |
en : Experimental Neurology
巻 382,
p. 114947,
発行日 2024-09-10
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| 旧ID |
56125 |
| 備考 |
The full-text file will be made open to the public on 10 September 2025 in accordance with publisher's 'Terms and Conditions for Self-Archiving' |
ExpNeu_382_114947.pdf (4.0 MB)
