| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Differential activation of astrocytes and microglia after spinal cord injury in the fetal rat |
|
言語 |
en |
| 作成者 |
Fujimoto, Yoshinori
Yamasaki, Takeshi
Tanaka, Nobuhiro
Mochizuki, Yu
Kajihara, Hiroki
Ikuta, Yoshikazu
Ochi, Mitsuo
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2006 Springer-Verlag. "The original publication is available at www.springerlin.com" |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Fetal Surgery |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Immunohistochemistry |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Astrocytes |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Microglia |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Spinal cord injury |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
|
|
内容記述 |
Background: Because the immature spinal cord was nerve growth permissive, we examined glial reactions that influence regeneration of the spinal cord in a fetal rat spinal cord injury model. Methods: Three, 7, 21, and 35 days after intrauterine surgery, offsprings were killed and the thoracic and lumbar spinal cords were carefully removed from the spinal column and then cut into 10 m longitudinal sections. These sections were stained with hematoxylin-eosin, anti-glial fibrillary acidic protein antibody (GFAP) as a marker of astrocytes, and anti-complement CR3 antibody (OX-42) as a marker of microglia. A cordotomy model in young adult rat was utilized as a control. Results: In the present study, collagen fibers and scar formation were seen in the severed spinal cords of mature rats, but scar formation was not seen in the fetal rat cordotomy group, regardless of spinal continuity. In the Control group, biological activity of GFAP-positive cells increased over time. In the fetal rat cordotomy model, activity elevated slightly immediately after cordotomy, and disappeared shortly thereafter. In the Control group, OX-42 positive macrophage-like cells proliferated over time. However, in the fetal rat cordotomy model, OX-42 positive macrophage-like cells were recognized on postoperative days 3 and 7, and then disappeared. At 5 mm from the cordotomy site, reactive microglia were recognized in the white matter of Control group spinal cords, but these microglia were not recognized in the fetal rat cordotomy model. Conclusions: In the present study, collagen fibers and scar formation were seen in the severed spinal cords of adult rats, but scar formation was not seen in the fetal rat cordotomy group. Lack of inflammation and scar formation thus appear advantageous for regeneration of the fetal spinal cord. Between fetal and mature rats, chronological changes in the immunohistochemical reactions of astrocytes and microglia following cordotomy were compared, and the results confirmed many differences. The results of the prese |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Springer |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1007/s00586-005-0933-3 |
| 関連情報 |
|
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|
識別子タイプ |
PMID |
|
|
関連識別子 |
16292632 |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1007/s00586-005-0933-3 |
| 収録物識別子 |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0940-6719 |
| 収録物識別子 |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10852035 |
| 開始ページ |
|
|
開始ページ |
223 |
| 書誌情報 |
European Spine Journal
European Spine Journal
巻 15,
号 2,
p. 223-233,
発行日 2006-02-02
|
| 旧ID |
17069 |
ESJ_15_223.pdf (2.5 MB)
