| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Effects of overexpression of basic helix-loop-helix transcription factor Dec1 on osteogenic and adipogenic differentiation of mesenchymal stem cells |
|
言語 |
en |
| 作成者 |
Iwata, Tomoyuki
Kawamoto, Takeshi
Sasabe, Eri
Miyazaki, Kazuko
Fujimotoa, Katsumi
Noshiro, Mitsuhide
Kurihara, Hidemi
Kato, Yukio
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| アクセス権 |
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|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2006 Elsevier GmbH. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Dec1 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Stra13 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Bhlhb2 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
DEC2 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
mesenchymal stem cell |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
osteogenesis |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
adipogenesis |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
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|
内容記述 |
We recently reported that forced expression of basic helix-loop-helix transcription factor Dec1 accelerated chondrogenic differentiation of mesenchymal stem cells (MSC) in pellet cultures (M. Shen, E. Yoshida, W. Yan, T. Kawamoto, K. Suardita, Y. Koyano, K. Fujimoto, M. Noshiro and Y. Kato, 2002. J. Biol. Chem. 277:50112-50120). Since MSC have multilineage differentiation potential, we investigated the roles of Dec1 in osteogenic and adipogenic differentiation of human bone marrow-derived MSC. After osteogenic induction of MSC in medium containing dexamethasone, β- glycerophosphate, and ascorbic acid, Dec1 expression gradually increased from day 5 to day 14, while expression levels of Dec1 mRNA markedly decreased on days 3 and 7 after adipogenic induction. Infection with adenovirus expressing Dec1 raised mRNA levels of several bone characteristic molecules such as osteopontin, PTH receptor and alkaline phosphatase, even in the absence of the osteogenic-induction medium, although it had little effect on Runx2 expression or calcification. In the osteogenic-induction medium, Dec1 overexpression enhanced the expression of osteopontin and alkaline phosphatase and induced matrix calcification. Knockdown of Dec1 with siRNA suppressed the expression of osteoblastic phenotype by the induced MSC. Using MSC cultures, we also confirmed that forced expression of Dec1 suppressed adipogenic differentiation. These findings suggest that Dec1 modulates osteogenic differentiation of MSC by inducing the expression of several, but not all, bone-related genes. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
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|
識別子タイプ |
DOI |
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|
関連識別子 |
10.1016/j.ejcb.2005.12.007 |
| 関連情報 |
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|
識別子タイプ |
PMID |
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|
関連識別子 |
16487626 |
| 関連情報 |
|
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.ejcb.2005.12.007 |
| 収録物識別子 |
|
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0171-9335 |
| 収録物識別子 |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00181272 |
| 開始ページ |
|
|
開始ページ |
423 |
| 書誌情報 |
European Journal of Cell Biology
European Journal of Cell Biology
巻 85,
号 5,
p. 423-431,
発行日 2006-03-03
|
| 旧ID |
17064 |
EurJCellBiol_85_423.pdf (1.7 MB)
