| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
A non-canonical function of Plk4 in centriolar satellite integrity and ciliogenesis through PCM1 phosphorylation |
|
言語 |
en |
| 作成者 |
Hori, Akiko
Barnouin, Karin
Snijders, Ambrosius P
Toda, Takashi
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
© 2016 The Authors. This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
centriolar satellites |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
centrosome |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
ciliogenesis |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
PCM1 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Plk4 |
| 内容記述 |
|
|
内容記述 |
Centrioles are the major constituents of the animal centrosome, in which Plk4 kinase serves as a master regulator of the duplication cycle. Many eukaryotes also contain numerous peripheral particles known as centriolar satellites. While centriolar satellites aid centriole assembly and primary cilium formation, it is unknown whether Plk4 plays any regulatory roles in centriolar satellite integrity. Here we show that Plk4 is a critical determinant of centriolar satellite organisation. Plk4 depletion leads to the dispersion of centriolar satellites and perturbed ciliogenesis. Plk4 interacts with the satellite component PCM1, and its kinase activity is required for phosphorylation of the conserved S372. The nonphosphorylatable PCM1 mutant recapitulates phenotypes of Plk4 depletion, while the phosphomimetic mutant partially rescues the dispersed centriolar satellite patterns and ciliogenesis in cells depleted of PCM1. We show that S372 phosphorylation occurs during the G1 phase of the cell cycle and is important for PCM1 dimerisation and interaction with other satellite components. Our findings reveal that Plk4 is required for centriolar satellite function, which may underlie the ciliogenesis defects caused by Plk4 dysfunction. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
EMBO Press |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.15252/embr.201541432 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
26755742 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.15252/embr.201541432 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1469-221X |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1469-3178 |
| 開始ページ |
|
|
開始ページ |
326 |
| 書誌情報 |
EMBO Reports
EMBO Reports
巻 17,
号 3,
p. 326-337,
発行日 2016-03-01
|
| 旧ID |
48783 |
EMBORep_17_326.pdf (2.0 MB)
