| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Protective effects of bacteriophage on experimental Lactococcus garvieae infection in yellowtail |
|
言語 |
en |
| 作成者 |
Nakai, Toshihiro
Sugimoto, R.
Park, K.-H.
Matsuoka, Satoru
Mori, Koh-ichiro
Nishioka, Toyohiro
Maruyama, Keigo
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 1999 Inter-Research. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Bacteriophage |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Lactococcus garvieae |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Enterococcus seriolicida |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Yellowtail |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Seriola quinqueradiata |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Phage-therapy |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Biological control |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
480 |
| 内容記述 |
|
|
内容記述 |
The present study describes the in vitro and in vivo survival of Lactococcus garvieae bacteriophages and the potential of the phage for controlling experimental L. garvieae infection in yellowtail. Anti-L. garvieae phages persisted well in various physicochemical (water temperature, salinity, pH) and biological (feed, serum and alimentary tract extracts of yellowtail) conditions, except for low acidity. In the in vivo, the phage PLgY-16 was detected in the spleens of yellowtail until 24 h after intraperitoneal (i.p.) injection, or the phage was recovered from the intestine of yellowtail 3 h after the oral administration of phage-impregnated feed but undetectable 10 h later. Simultaneous administration of live L. garvieae and phage enhanced recovery of the phage from the spleen or intestine. The survival rate was much higher in yellowtail that received i.p. injection of the phage after i.p. challenge with L. garvieae, compared with that of control fish without phage injection. When fish were i.p.-injected with phage at different hours after L. garvieae challenge, higher protective effects were demonstrated in fish that received phage treatment at the earlier time. Protection was also obtained in yellowtail receiving phage-impregnated feed, in which fish were challenged by an anal intubation with L. garvieae. Anal-intubated L. garvieae were detected constantly in the spleens of the control fish, while they were detected sporadically and disappeared from the phage-treated fish 48 h later. On the other hand, orally administered phage was detected at high plaque-forming units from the intestines and spleens of the phage-treated fish until 48 h later. These results indicate that intraperitoneally or orally administered anti-L. garvieae phage prevented fish from experimental L. garvieae infection, suggesting potential use of the phage for controlling the disease. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Inter-Research |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.3354/dao037033 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.3354/dao037033 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0177-5103 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1616-1580 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10443976 |
| 開始ページ |
|
|
開始ページ |
33 |
| 書誌情報 |
Diseases of Aquatic Organisms
Diseases of Aquatic Organisms
巻 37,
号 1,
p. 33-41,
発行日 1999-06-23
|
| 旧ID |
25657 |
dao037p033.pdf (788.4 KB)
