| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
16S rRNA targeted RT-PCR for the detection of Vibrio penaeicida, the pathogen of cultured kuruma prawn Penaeus japonicus. |
|
言語 |
en |
| 作成者 |
Genmoto, K.
Nishizawa, Toyohiko
Nakai, Toshihiro
Muroga, Kiyokuni
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 1996 Inter-Research. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Vibrio penaeicida |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
rRNA |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
PCR Kuruma prawn |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Vibriosis |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
480 |
| 内容記述 |
|
|
内容記述 |
Vibrio penaeicida is the causative bacterium of vibriosis in cultured kuruma prawn Penaeus japonicus in Japan. To develop a specific and sensitive method for the detection of the pathogen, a species-specific sequence in the 16S rRNA of V. penaeicida was determined and a polymerase chain reaction (PCR)-based method was devised on the basis of the sequence. Prior to sequencing, a part of the variable regions of the 16S rRNA was amplified by using primers designed from 2 conserved regions according to previously reported data on Vibrionaceae. The region of the 16S rRNA (nucleotide numbers 440 to 490 in Escherichia coli 16S rRNA) obtained by this procedure was found to be species-specific for V. penaeicida. It was confirmed that PCR and RT (reverse transcription)-PCR amplifications with a sense primer designed from the V. penaeicida-specific sequence were both able to differentiate V. penaeicida from other prawn-pathogenic vibrios. 16S rRNA-targeted RT-PCR was demonstrated to have 100 times higher sensitivity than 16S rDNA-targeted PCR and 10 fg of total nucleic acids extracted from cultured bacterial cells was sufficient to yield the visible fragment in gel electrophoresis. These results indicate that RT-PCR amplification with this primer is useful for specific and sensitive detection of V. penaeicida. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Inter-Research |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.3354/dao024185 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.3354/dao024185 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0177-5103 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1616-1580 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10443976 |
| 開始ページ |
|
|
開始ページ |
185 |
| 書誌情報 |
Diseases of Aquatic Organisms
Diseases of Aquatic Organisms
巻 24,
号 3,
p. 185-189,
発行日 1996-03-07
|
| 旧ID |
25653 |
dao024p185.pdf (551.9 KB)
