| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Molecular biological approaches to the accumulation and reduction of vanadium by ascidians |
|
言語 |
en |
| 作成者 |
Michibata, Hitoshi
Yamaguchi, Nobuo
Uyama, Taro
Ueki, Tatsuya
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2002 Elsevier Science B.V. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Ascidian |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Tunicate |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Vanadium |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Accumulation |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Reduction |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Metalbinding protein |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
430 |
| 内容記述 |
|
|
内容記述 |
About 90 years ago, Henze discovered high levels of vanadium in the blood (coelomic) cells of an ascidian collected from the Bay of Naples. His discovery attracted the interdisciplinary attention of chemists, physiologists, and biochemists. Two decades ago, we quantified the vanadium levels in several ascidian tissues definitively using neutron-activation analysis and revealed that some species in the family Ascidiidae accumulate vanadium at concentrations in excess of 350 mM, corresponding to about 107 times that found in seawater. Vanadium accumulated is reduced to the +3 oxidation state via the +4 oxidation state and stored in vacuoles of vanadocytes (vanadium-containing blood cells) where high levels of protons and sulfate are also contained. To investigate this unusual phenomenon, we isolated several proteins and genes that are expressed in vanadocytes. To date, three types of vanadium-binding protein, designated as Vanabins, have been isolated, with molecular masses of 12.5, 15, and 16 kDa, along with the cDNAs encoding these proteins. In addition, four types of enzyme related to the pentose phosphate pathway that produces NADPH were revealed to be located in vanadocytes. The pentose phosphate pathway participates in the reduction of vanadium(V) to vanadium(IV). The cDNA for each of the vacuolar-type H+-ATPase (V-ATPase) A, B, C, and D subunits, which are located on the vacuolar membranes of vanadocytes, has been isolated and analyzed. V-ATPase generates a proton-motive force, and is thought to provide the energy for vanadium accumulation.To clarify the entire mechanism involved in the accumulation and reduction, much more genes and proteins expressed in the blood cells need to be systematically identified. Thus, we have performed an expressed sequence tag (EST) analysis of blood cells and have established the functional assay system to elucidate the functions of genes and proteins obtained from ascidian blood cells. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier Science B.V. |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/S0010-8545(02)00278-3 |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/S0010-8545(02)00278-3 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0010-8545 |
| 開始ページ |
|
|
開始ページ |
41 |
| 書誌情報 |
Coordination chemistry reviews
Coordination chemistry reviews
巻 237,
号 1-2,
p. 41-51,
発行日 2003
|
| 旧ID |
21 |
CoordChemRev_234_41-51_2003.pdf (538.6 KB)
