| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Molecular cloning and effect of c-fos mRNA on pharmacological stimuli in the goldfish brain |
|
言語 |
en |
| 作成者 |
Fujiwara, Yukichi
Kozono, Keina
Esaka, Muneharu
Iijima, Noriaki
Nagamatsu, Yasunori
Yoshida, Masayuki
Uematasu, Kazumasa
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
c-fos |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
immedicate early gene |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
goldfish |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
kainic acid |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
660 |
| 内容記述 |
|
|
内容記述 |
c-fos is an immediate early gene, and is rapidly and transiently induced in neurons of the central nervous system according to their activities. To investigate neuronal activities in the brain of the goldfish (Carassius auratus), we considered that expression of c-fos mRNA would be an available marker for the neuronal activities. Therefore, we firstly isolated a cDNA clone encoding c-Fos from the goldfish brain by RT-PCR and RACE methods. A full length cDNA of the goldfish c-fos was composed of 1,044 bp open reading frame. The amino acid sequence of the goldfish c-Fos was approximately 56-90 0dentical to those of teleost fish c-Fos. Northern blot analysis showed that the expression of c-fos mRNA was rapidly and transiently induced in the brain of the goldfish by the intraperitoneal administration of kainic acid. We also showed that the identification of the c-fos mRNA expression site by in situ hybridization will be able to use as an anatomical marker for identification of the activated neuronal region in the goldfish brain. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier Inc. |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.cbd.2005.12.005 |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.cbd.2005.12.005 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12142777 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1744-117X |
| 開始ページ |
|
|
開始ページ |
253 |
| 書誌情報 |
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics
巻 1,
号 2,
p. 253-259,
発行日 2006-06
|
| 旧ID |
17245 |
CBPD_1_253.pdf (371.9 KB)
