| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
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|
タイトル |
Ontogeny of gene expression of group IB phospholipase A(2) isoforms in the red sea bream, Pagrus (Chrysophrys) major |
|
言語 |
en |
| 作成者 |
Fujikawa, Yukichi
Shimokawa, Mariko
Satoh, Fumi
Satoh, Osamu
Yoshioka, Daisuke
Aida, Satoshi
Uematsu, Kazumasa
Iijima, Noriaki
|
| アクセス権 |
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|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
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権利情報 |
(c) 2011 Elsevier Inc. All rights reserved. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Adult |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Group IB phospholipase A(2) |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Isoform |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
In situ hybridization |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
Larva |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Pagrus major |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Teleost |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
460 |
| 内容記述 |
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|
内容記述 |
The red sea bream (Pagrus major) was previously found to express mRNAs for two group IB phospholipase A(2) (PLA(2)) isoforms, DE-1 and DE-2, in the digestive organs, including the hepatopancreas, pyloric caeca, and intestine. To characterize the ontogeny of the digestive function of these PLA(2)s, the present study investigated the localization and expression of DE-1 and DE-2 PLA(2) genes in red sea bream larvae/juveniles and immature adults, by in situ hybridization. In the adults, DE-1 PLA(2) mRNA was expressed in pancreatic acinar cells. By contrast, DE-2 PLA(2) mRNA was detected not only in digestive tissues, such as pancreatic acinar cells, gastric glands of the stomach, epithelial cells of the pyloric caeca, and intestinal epithelial cells, but also in non-digestive ones, including cardiac and lateral muscle fibers and the cytoplasm of the oocytes. In the larvae, both DE-1 and DE-2 PLA(2) mRNAs first appeared in pancreatic tissues at 3 days post-hatching (dph) and in intestinal tissue at 1 dph, and expression levels for both gradually increased after this point. In the juvenile stage at 32 dph, DE-1 PLA(2) mRNA was highly expressed in pancreatic tissue, and DE-2 PLA(2) mRNA was detected in almost all digestive tissues, including pancreatic tissue, gastric glands, pyloric caeca, and intestine, including the myomere of the lateral muscles. In conclusion, both DE-1 and DE-2 PLA(2) mRNAs are already expressed in the digestive organs of red sea bream larvae before first feeding, and larvae will synthesize both DE-1 and DE-2 PLA(2) proteins. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier Science INC |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
10.1016/j.cbpa.2011.10.022 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.cbpa.2011.10.022 |
| 収録物識別子 |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1095-6433 |
| 収録物識別子 |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11183804 |
| 開始ページ |
|
|
開始ページ |
185 |
| 書誌情報 |
Comparative Biochemistry and Physiology Part A : Molecular & Integrative Physiology
Comparative Biochemistry and Physiology Part A : Molecular & Integrative Physiology
巻 161,
号 2,
p. 185-192,
発行日 2011
|
| 旧ID |
34828 |
CompBiochemPhysiolA_161_185.pdf (17.2 MB)
