| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Effects of hypergravity environments on amphibian development, gene expression and apoptosis |
|
言語 |
en |
| 作成者 |
Kawakami, Satomi
Kashiwagi, Keiko
Furuno, Nobuaki
Yamashita, Masamichi
Kashiwagi, Akihiko
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2006 Elsevier Ltd. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Space environments |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Hypergravity |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Xenopus laevis |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Amphibian development |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Gene expression |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Apoptosis |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Developmental retardation |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Morphological abnormalities |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
460 |
| 内容記述 |
|
|
内容記述 |
This study investigates how rearing under conditions of hypergravity affects amphibian development, Xotx2 and Xag1 gene expression and apoptosis. Uncleaved Xenopus laevis eggs 20 min after insemination, 2 cell stage embryos, and gastrula stage embryos were raised at 2G and 5G, while controls were raised in normal gravity. Apoptosis in brain and eye inner structures of hatching embryos was scored using the TUNEL staining method, and gene expression in tail-bud embryos was analyzed by whole-mount in situ hybridization. Results showed that: (1) 5G retarded the development of eggs and embryos and induced microcephaly and microphthalmia. (2) 5G suppressed the expression of the two genes, Xotx2 (involved in fore- and midbrain and eye development) and Xag1 (regulating cement gland formation). (3) Eggs and 2 cell stage embryos raised at 5G showed a greater extent of brain and eye apoptosis compared with controls, while those raised at 2G showed no significant difference. These findings suggest that high gravity suppresses certain gene functions and induces abnormal apoptosis in brain and eyes, resulting in developmental retardation and various morphological abnormalities. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.cbpa.2006.05.006 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
16807024 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.cbpa.2006.05.006 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1095-6433 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11183804 |
| 開始ページ |
|
|
開始ページ |
65 |
| 書誌情報 |
Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology
巻 145,
号 1,
p. 65-72,
発行日 2006-09
|
| 旧ID |
17056 |
CBP_145_65.pdf (3.6 MB)
