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Characterization of the mechanism of interaction between α1-acid glycoprotein and lipid membranes by vacuum-ultraviolet circular-dichroism spectroscopy

https://hiroshima.repo.nii.ac.jp/records/2006651
https://hiroshima.repo.nii.ac.jp/records/2006651
73a4ddbe-4ecc-487f-ac41-858373cace75
名前 / ファイル ライセンス アクション
Chirality_32_594.pdf Chirality_32_594.pdf (571.0 KB)
Item type デフォルトアイテムタイプ_(フル)(1)
公開日 2023-03-18
タイトル
タイトル Characterization of the mechanism of interaction between α1-acid glycoprotein and lipid membranes by vacuum-ultraviolet circular-dichroism spectroscopy
言語 en
作成者 Matsuo, Koichi

× Matsuo, Koichi

en Matsuo, Koichi

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Kumashiro, Munehiro

× Kumashiro, Munehiro

en Kumashiro, Munehiro

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Gekko, Kunihiko

× Gekko, Kunihiko

en Gekko, Kunihiko

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
権利情報 This is the peer reviewed version of the following article: Matsuo, K, Kumashiro, M, Gekko, K. Characterization of the mechanism of interaction between α1-acid glycoprotein and lipid membranes by vacuum-ultraviolet circular-dichroism spectroscopy. Chirality. 2020; 32: 594– 604, which has been published in final form at https://doi.org/10.1002/chir.23208. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
権利情報
権利情報 This is not the published version. Please cite only the published version. この論文は出版社版ではありません。引用の際には出版社版をご確認、ご利用ください。
主題
主題Scheme Other
主題 electrostatic and hydrophobic interactions
主題
主題Scheme Other
主題 membrane-bound conformation
主題
主題Scheme Other
主題 secondary structure of protein
主題
主題Scheme Other
主題 synchrotron radiation circular dichroism
主題
主題Scheme Other
主題 α1-acid glycoprotein
内容記述
内容記述 α1-Acid glycoprotein (AGP) interacts with lipid membranes as a peripheral membrane protein so as to decrease the drug-binding capacity accompanying the β→α conformational change that is considered a protein-mediated uptake mechanism for releasing drugs into membranes or cells. This study characterized the mechanism of interaction between AGP and lipid membranes by measuring the vacuum-ultraviolet circular-dichroism (VUVCD) spectra of AGP down to 170 nm using synchrotron radiation in the presence of five types of liposomes whose constituent phospholipid molecules have different molecular characteristics in the head groups (e.g., different net charges). The VUVCD analysis showed that the α-helix and β-strand contents and the numbers of segments of AGP varied with the constituent phospholipid molecules of liposomes, while combining VUVCD data with a neural-network method predicted that these membrane-bound conformations comprised several common long helix and small strand segments. The amino-acid composition of each helical segment of the conformations indicated that amphiphilic and positively charged helices formed at the N- and C-terminal regions of AGP, respectively, were candidate sites for the membrane interaction. The addition of 1 M sodium chloride shortened the C-terminal helix while having no effect on the length of the N-terminal one. These results suggest that the N- and C-terminal helices can interact with the membrane via hydrophobic and electrostatic interactions, respectively, demonstrating that the liposome-dependent conformations of AGP analyzed using VUVCD spectroscopy provide useful information for characterizing the mechanism of interaction between AGP and lipid membranes.
言語 en
内容記述
内容記述タイプ Other
内容記述 This work was financially supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (numbers 19K06587 and 15K07028).
内容記述
内容記述タイプ Other
内容記述 [This article is part of the Special Issue: In honor and memory of Prof. Koji Nakanishi. See the first articles for this special issue previously published in Volumes 31:12, 32:3, and 32:4. More special articles will be found in this issue as well as in those to come.]
出版者
出版者 Wiley
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
出版タイプ
出版タイプ AO
出版タイプResource http://purl.org/coar/version/c_b1a7d7d4d402bcce
関連情報
識別子タイプ DOI
関連識別子 10.1002/chir.23208
関連情報
識別子タイプ PMID
関連識別子 32125028
関連情報
識別子タイプ DOI
関連識別子 https://doi.org/10.1002/chir.23208
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0899-0042
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 1520-636X
開始ページ
開始ページ 594
書誌情報 Chirality
Chirality

巻 32, 号 5, p. 594-604, 発行日 2020-04-16
旧ID 51083
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