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One fold, two functions: cytochrome P460 and cytochrome c′-β from the methanotroph Methylococcus capsulatus (Bath)

https://hiroshima.repo.nii.ac.jp/records/2006621
https://hiroshima.repo.nii.ac.jp/records/2006621
95cbf6d0-c0c1-49fe-876d-90d6ce80c06b
名前 / ファイル ライセンス アクション
ChemSci_10_3031.pdf ChemSci_10_3031.pdf (1.2 MB)
Item type デフォルトアイテムタイプ_(フル)(1)
公開日 2023-03-18
タイトル
タイトル One fold, two functions: cytochrome P460 and cytochrome c′-β from the methanotroph Methylococcus capsulatus (Bath)
言語 en
作成者 Adams, Hannah R.

× Adams, Hannah R.

en Adams, Hannah R.

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Krewson, Callie

× Krewson, Callie

en Krewson, Callie

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Vardanega, Jenny E.

× Vardanega, Jenny E.

en Vardanega, Jenny E.

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Fujii, Sotaro

× Fujii, Sotaro

en Fujii, Sotaro

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Moreno, Tadeo

× Moreno, Tadeo

en Moreno, Tadeo

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Chicano

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en Chicano

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Sambongi, Yoshihiro

× Sambongi, Yoshihiro

en Sambongi, Yoshihiro

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Svistunenko, Dimitri

× Svistunenko, Dimitri

en Svistunenko, Dimitri

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Paps, Jordi

× Paps, Jordi

en Paps, Jordi

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Andrew, Colin R.

× Andrew, Colin R.

en Andrew, Colin R.

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Hough, Michael A.

× Hough, Michael A.

en Hough, Michael A.

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
権利情報 © The Royal Society of Chemistry 2019
権利情報
権利情報 This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
内容記述
内容記述 Nature is adept at utilising highly similar protein folds to carry out very different functions, yet the mechanisms by which this functional divergence occurs remain poorly characterised. In certain methanotrophic bacteria, two homologous pentacoordinate c-type heme proteins have been identified: a cytochrome P460 (cyt P460) and a cytochrome c0-b (cyt cp-b). Cytochromes P460 are able to convert hydroxylamine to nitrous oxide (N2O), a potent greenhouse gas. This reactivity is similar to that of hydroxylamine oxidoreductase (HAO), which is a key enzyme in nitrifying and methanotrophic bacteria. Cyt P460 and HAO both have unusual protein-heme cross-links, formed by a Tyr residue in HAO and a Lys in cyt P460. In contrast, cyts cp-b (the only known cytochromes c0 with a b-sheet fold) lack this crosslink and appears to be optimized for binding non-polar molecules (including NO and CO) without enzymatic conversion. Our bioinformatics analysis supports the proposal that cyt cp-b may have evolved from cyt P460 via a gene duplication event. Using high-resolution X-ray crystallography, UV-visible absorption, electron paramagnetic resonance (EPR) and resonance Raman spectroscopy, we have characterized the overall protein folding and active site structures of cyt cp-b and cyt P460 from the obligate methanotroph, Methylococcus capsulatus (Bath). These proteins display a similar b-sheet protein fold, together with a pattern of changes to the heme pocket regions and localised tertiary structure that have converted a hydroxylamine oxidizing enzyme into a gas-binding protein. Structural comparisons provide insights relevant to enzyme redesign for synthetic enzymology and engineering of gas sensor proteins. We also show the widespread occurrence of cyts cp-b and characterise their phylogeny.
言語 en
内容記述
内容記述タイプ Other
内容記述 CRA acknowledges funding from the National Science Foundation (grant MCB 1411963). TMC was supported by Leverhulme Trust award RPG-2014-355 to MAH.
出版者
出版者 Royal Society of Chemistry
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
関連情報
関連名称 Electronic supplementary information (ESI) available. See DOI: 10.1039/c8sc05210g
関連情報
識別子タイプ DOI
関連識別子 10.1039/C8SC05210G
関連情報
識別子タイプ DOI
関連識別子 https://doi.org/10.1039/C8SC05210G
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 2041-6520
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 2041-6539
開始ページ
開始ページ 3031
書誌情報 Chemical Science
Chemical Science

巻 10, 号 10, p. 3031-3041, 発行日 2019-01-21
旧ID 48209
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