| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Imaging and mapping of mouse bone using MALDI-imaging mass spectrometry |
|
言語 |
en |
| 作成者 |
Fujino, Yoko
Minamizaki, Tomoko
Yoshioka, Hirotaka
Okada, Mitsugi
Yoshiko, Yuji
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
© 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Matrix-assisted laser desorption/ionization-imaging mass spectrometry |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Tissue cryosection |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Bone |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Fixation |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Decalcification |
| 内容記述 |
|
|
内容記述 |
Matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) is an advanced method used globally to analyze the distribution of biomolecules on tissue cryosections without any probes. In bones, however, hydroxyapatite crystals make it difficult to determine the distribution of biomolecules using MALDI-IMS. Additionally, there is limited information regarding the use of this method to analyze bone tissues. To determine whether MALDI-IMS analysis of bone tissues can facilitate comprehensive mapping of biomolecules in mouse bone, we first dissected femurs and tibiae from 8-week-old male mice and characterized the quality of multiple fixation and decalcification methods for preparation of the samples. Cryosections were mounted on indium tin oxide-coated glass slides, dried, and then a matrix solution was sprayed on the tissue surface. Images were acquired using an iMScope at a mass-to-charge range of 100–1000. Hematoxylin-eosin, Alcian blue, Azan, and periodic acid-Schiff staining of adjacent sections was used to evaluate histological and histochemical features. Among the various fixation and decalcification conditions, sections from trichloroacetic acid-treated samples were most suitable to examine both histology and comprehensive MS images. However, histotypic MS signals were detected in all sections. In addition to the MS images, phosphocholine was identified as a candidate metabolite. These results indicate successful detection of biomolecules in bone using MALDI-IMS. Although analytical procedures and compositional adjustment regarding the performance of the device still require further development, IMS appears to be a powerful tool to determine the distribution of biomolecules in bone tissues. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.bonr.2016.09.004 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
28580397 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.bonr.2016.09.004 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2352-1872 |
| 開始ページ |
|
|
開始ページ |
280 |
| 書誌情報 |
Bone Reports
Bone Reports
巻 5,
p. 280-285,
発行日 2016-12
|
| 旧ID |
48979 |
BoneReports_5_280.pdf (2.2 MB)
