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Imaging and mapping of mouse bone using MALDI-imaging mass spectrometry

https://hiroshima.repo.nii.ac.jp/records/2006527
https://hiroshima.repo.nii.ac.jp/records/2006527
aef3fe71-3e1d-4315-8c25-262db0c3b8b5
名前 / ファイル ライセンス アクション
BoneReports_5_280.pdf BoneReports_5_280.pdf (2.2 MB)
Item type デフォルトアイテムタイプ_(フル)(1)
公開日 2023-03-18
タイトル
タイトル Imaging and mapping of mouse bone using MALDI-imaging mass spectrometry
言語 en
作成者 Fujino, Yoko

× Fujino, Yoko

en Fujino, Yoko

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Minamizaki, Tomoko

× Minamizaki, Tomoko

en Minamizaki, Tomoko

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Yoshioka, Hirotaka

× Yoshioka, Hirotaka

en Yoshioka, Hirotaka

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Okada, Mitsugi

× Okada, Mitsugi

en Okada, Mitsugi

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Yoshiko, Yuji

× Yoshiko, Yuji

en Yoshiko, Yuji

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
権利情報 © 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
主題
主題Scheme Other
主題 Matrix-assisted laser desorption/ionization-imaging mass spectrometry
主題
主題Scheme Other
主題 Tissue cryosection
主題
主題Scheme Other
主題 Bone
主題
主題Scheme Other
主題 Fixation
主題
主題Scheme Other
主題 Decalcification
内容記述
内容記述 Matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) is an advanced method used globally to analyze the distribution of biomolecules on tissue cryosections without any probes. In bones, however, hydroxyapatite crystals make it difficult to determine the distribution of biomolecules using MALDI-IMS. Additionally, there is limited information regarding the use of this method to analyze bone tissues. To determine whether MALDI-IMS analysis of bone tissues can facilitate comprehensive mapping of biomolecules in mouse bone, we first dissected femurs and tibiae from 8-week-old male mice and characterized the quality of multiple fixation and decalcification methods for preparation of the samples. Cryosections were mounted on indium tin oxide-coated glass slides, dried, and then a matrix solution was sprayed on the tissue surface. Images were acquired using an iMScope at a mass-to-charge range of 100–1000. Hematoxylin-eosin, Alcian blue, Azan, and periodic acid-Schiff staining of adjacent sections was used to evaluate histological and histochemical features. Among the various fixation and decalcification conditions, sections from trichloroacetic acid-treated samples were most suitable to examine both histology and comprehensive MS images. However, histotypic MS signals were detected in all sections. In addition to the MS images, phosphocholine was identified as a candidate metabolite. These results indicate successful detection of biomolecules in bone using MALDI-IMS. Although analytical procedures and compositional adjustment regarding the performance of the device still require further development, IMS appears to be a powerful tool to determine the distribution of biomolecules in bone tissues.
言語 en
出版者
出版者 Elsevier
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
関連情報
識別子タイプ DOI
関連識別子 10.1016/j.bonr.2016.09.004
関連情報
識別子タイプ PMID
関連識別子 28580397
関連情報
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.bonr.2016.09.004
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 2352-1872
開始ページ
開始ページ 280
書誌情報 Bone Reports
Bone Reports

巻 5, p. 280-285, 発行日 2016-12
旧ID 48979
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