| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Characterization of established cementoblast-like cell lines from human cementum-lining cells in vitro and in vivo |
|
言語 |
en |
| 作成者 |
Kitagawa, Masae
Tahara, Hidetoshi
Kitagawa, Shoji
Oka, Hiroko
Kudo, Yasusei
Sato, Sunao
Ogawa, Ikuko
Miyauchi, Mutsumi
Takata, Takashi
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2006 Elsevier Ltd. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
cementoblast |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
periodontal ligament |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
hTERT |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
immortalization, differentiation |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
|
|
内容記述 |
To Study cellular characteristics of human cementoblasts using a cellular model is important for understanding the mechanisms of homeostasis and regeneration of periodontal tissues. However, at present no immortalized human cementoblast cell line has been established due to limitation of the life span. In the present study, therefore, we attempted to establish human cementoblast-like cell lines by transfection with telomerase catalytic subunit hTERT gene. Two stable clones (HCEM-1 and -2) with high telomerase activity were obtained and they grew over 200 population doublings without significant growth retardation. The expression of mRNA for differentiation markers, type I collagen, alkaline phosphatase (ALP), runt related transcription factor 2, osteocalcin and bone sialoprotein was revealed in these clones by RT-PCR. Moreover, these cells showed high ALP activity and calcified nodule formation in vitro. Interestingly, HCEM-2 showed cementum like formation on the surface of hydroxyapatites granules by subcutaneous transplantation into immunodeficient mice with hydroxyapatite granules. Thus, we established human cementblast-like cell lines with high mineralization activity. We suggest that HCEM cell lines can be useful cell models for investigating the characteristics of human cementoblasts. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.bone.2006.05.022 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
16857433 |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.bone.2006.05.022 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
8756-3282 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10506985 |
| 開始ページ |
|
|
開始ページ |
1035 |
| 書誌情報 |
Bone
Bone
巻 39,
号 5,
p. 1035-1042,
発行日 2006-11
|
| 旧ID |
17042 |
Bone_39_1035.pdf (542.8 KB)
