| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Synergistic oligodeoxynucleotide strongly promotes CpG-induced interleukin-6 production |
|
言語 |
en |
| 作成者 |
Nigar, Shireen
Yamamoto, Yoshinari
Okajima, Takuma
Shigemori, Suguru
Sato, Takashi
Ogita, Tasuku
Shimosato, Takeshi
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
© The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
LGG |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
CpG-ODN |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
iSN-ODN |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
IL-6 |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Synergistic effect |
| 内容記述 |
|
|
内容記述 |
[Background] :Bacterial genomes span a significant portion of diversity, reflecting their adaptation strategies; these strategies include nucleotide usage biases that affect chromosome configuration. Here, we explore an immuno-synergistic oligodeoxynucleotide (iSN-ODN, named iSN34), derived from Lactobacillus rhamnosusGG (LGG) genomic sequences, that exhibits a synergistic effect on immune response to CpG-induced immune activation. [Methods]: The sequence of iSN34 was designed based on the genomic sequences of LGG. Pathogen-free mice were purchased from Japan SLC and maintained under temperature- and light-controlled conditions. We tested the effects of iSN34 exposure in vitro and in vivo by assessing effects on mRNA expression, protein levels, and cell type in murine splenocytes. [Results]: We demonstrate that iSN34 has a significant stimulatory effect when administered in combination with CpGODN, yielding enhanced interleukin (IL)-6 expression and production. IL-6 is a pleotropic cytokine that has been shown to prevent epithelial apoptosis during prolonged inflammation. [Conclusions]: Our results are the first report of a bacterial-DNA-derived ODN that exhibits immune synergistic activity.The potent over-expression of IL-6 in response to treatment with the combination of CpG ODN and iSN34 suggests anew approach to immune therapy.This finding may lead to novel clinical strategies for the prevention or treatment of dysfunctions of the innate and adaptive immune systems. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
This work was supported by A-STEP (Adaptable and Seamless Technology Transfer Program through Target-driven R&D). |
| 出版者 |
|
|
出版者 |
BioMed Central Ltd |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1186/s12865-017-0227-7 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
28978323 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1186/s12865-017-0227-7 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1471-2172 |
| 開始ページ |
|
|
開始ページ |
44 |
| 書誌情報 |
BMC Immunology
BMC Immunology
巻 18,
p. 44,
発行日 2017-10-04
|
| 旧ID |
48507 |
bmci_18_44.pdf (1.1 MB)
