| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
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|
タイトル |
Surface plasmon resonance-biosensor detects the diversity of responses against epidermal growth factor in various carcinoma cell lines |
|
言語 |
en |
| 作成者 |
Hiragun, Takaaki
Yanase, Yuhki
Kose, Kazuhiro
Kawaguchi, Tomoko
Uchida, Kazue
Tanaka, Shinji
Hide, Michihiro
|
| アクセス権 |
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|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
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|
権利情報 |
(c) 2011 Elsevier B.V. All rights reserved. |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
Biosensor |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Surface plasmon resonance |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Living cell |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Cancer |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Epidermal growth factor |
| 主題 |
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|
主題Scheme |
Other |
|
主題 |
Diagnosis |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
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|
内容記述 |
Surface plasmon resonance (SPR) biosensor detects intracellular signaling events as a change of the angle of resonance (AR). We previously reported that the activation of epidermal growth factor receptor (EGFR) on keratinocytes causes a unique triphasic change of AR, whereas the activation of other receptors, such as IgE receptor and adenosine A3 receptor on mast cells, causes a transient monophasic increase of AR. To study the mechanism of AR changes induced by EGFR activation, we introduced wild and mutated EGFR cDNAs into Chinese hamster ovary (CHO) cells and analyzed changes of AR in response to EGF. CHO cells expressing wild-type EGFR showed a triphasic change of AR, whereas cells expressing kinase-dead EGFR (K721 M) showed minimum change of AR. A phosphatidylinositol 3-kinase inhibitor, wortmannin, attenuated the third phase of AR change in CHO cells expressing wild-type EGFR. The pattern of AR change was independent on the concentration of EGF. We also analyzed changes of AR with a nontumorigenic keratinocyte cell line, HaCaT, and several cell lines of carcinoma to explore the feasibility of SPR biosensor as a tool for clinical diagnosis. The activation of HaCaT cells and one out of six carcinoma cell lines showed a full triphasic change of AR. In contrast, five out of the six cell lines showed mono- or bi-phasic change of AR. These results suggest that EGF induces the SPR signals via the phosphorylation of EGFR, and provide a possibility that the SPR biosensor could be applied to the real-time detection and diagnosis of malignant tumors. |
|
言語 |
en |
| 出版者 |
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|
出版者 |
Elsevier Advanced Technology |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.bios.2011.12.004 |
| 関連情報 |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
http://dx.doi.org/10.1016/j.bios.2011.12.004 |
| 収録物識別子 |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0956-5663 |
| 収録物識別子 |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10739666 |
| 開始ページ |
|
|
開始ページ |
202 |
| 書誌情報 |
Biosensors & Bioelectronics
Biosensors & Bioelectronics
巻 32,
号 1,
p. 202-207,
発行日 2012
|
| 旧ID |
34831 |
BiosensBioelectron_32_202.pdf (655.1 KB)
