| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Development of an optical fiber SPR sensor for living cell activation |
|
言語 |
en |
| 作成者 |
Yanase, Yuhki
Araki, Atsunori
Suzuki, Hidenori
Tsutsui, Tomoko
Kimura, Tatsuo
Okamoto, Keishi
Nakatani, Tatsuyuki
Hiragun, Takaaki
Hide, Michihiro
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2009 Elsevier B.V. All rights reserved. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Biosensor |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Surface plasmon resonance |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Living cell |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Optical fiber |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Mast cells |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
|
|
内容記述 |
Surface plasmon resonance (SPR) sensors provide a useful means to study the interactions of biological molecules and the reaction of living cells on a sensor chip. However, conventional SPR sensors are bulky. expensive and complicated to use as common diagnostic equipment. In this study, we developed a relatively small and simple SPR system. using optical fibers of 250 pm diameter to detect the activation of living cells attached to the fiber tip. For this system, the core of 200 pm diameter with I cm length of an optical fiber was coated by gold film with 50 nm thickness to cause plasmon resonance. The light provided by a white LED and attenuated due to a SPR phenomenon in the sensor part was detected and analyzed using a spectrum detector. The difference in solvents with various refractive indexes and protein-bindings to the sensor tip was detected with sufficient sensitivity. Moreover, it detected a sustained increase of AR in a real-time manner, when RBL-2H3 mast cells were fixed onto the fiber tip and stimulated by an antigen. This small fiber SPR system might serve as a useful toot for various clinical examinations either within or outside the body. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier Advanced Technology |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.bios.2009.09.042 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.bios.2009.09.042 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0956-5663 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10739666 |
| 開始ページ |
|
|
開始ページ |
1244 |
| 書誌情報 |
Biosensors & Bioelectronics
Biosensors & Bioelectronics
巻 25,
号 5,
p. 1244-1247,
発行日 2010-01-15
|
| 旧ID |
29242 |
BiosensBioelectron_25_1244.pdf (226.8 KB)
