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Living cell positioning on the surface of gold film for SPR analysis

https://hiroshima.repo.nii.ac.jp/records/2006495
https://hiroshima.repo.nii.ac.jp/records/2006495
8b5b8c9e-5e64-42f6-828d-fc3e95ac3445
名前 / ファイル ライセンス アクション
BB_23_562.pdf BB_23_562.pdf (836.4 KB)
Item type デフォルトアイテムタイプ_(フル)(1)
公開日 2023-03-18
タイトル
タイトル Living cell positioning on the surface of gold film for SPR analysis
言語 en
作成者 Yanase, Yuhki

× Yanase, Yuhki

en Yanase, Yuhki

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Suzuki, Hidenori

× Suzuki, Hidenori

en Suzuki, Hidenori

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Tsutsui, Tomoko

× Tsutsui, Tomoko

en Tsutsui, Tomoko

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Uechi, Ichiro

× Uechi, Ichiro

en Uechi, Ichiro

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Hiragun, Takaaki

× Hiragun, Takaaki

en Hiragun, Takaaki

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Mihara, Shoji

× Mihara, Shoji

en Mihara, Shoji

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Hide, Michihiro

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en Hide, Michihiro

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
権利情報 Copyright(c) 2007 Elsevier Advanced Technology
主題
主題Scheme Other
主題 biosensor
主題
主題Scheme Other
主題 surface plasmon resonance
主題
主題Scheme Other
主題 non-adherent cells
主題
主題Scheme Other
主題 cell fixation
主題
主題Scheme Other
主題 recovery
主題
主題Scheme NDC
主題 490
内容記述
内容記述 Living cell reactions are detected as changes of the angle of resonance (AR) for surface plasmon resonance (SPR). Since SPR reflects the events in the field of evanescence, cells need to be fixed on the sensor chip. In this study, we developed methods to fix living cells on a gold surface and to recover adherent cells from the culture dish, preserving their functions to be analyzed by SPR. Human basophils and B cells were fixed to the sensor chip by a biocompatible anchor for cell membranes (alpha-succinimidyloxysuccinyl omega-oleyloxy polyoxyethylene), aminoalkanethiol (cyteamine, 8-amino octanethiol) or an amino-reactive cross-linker (dithiobis [succinimidylpropionate]). They showed an increase of AR in response to various stimuli. RBL-2H3 cells, which firmly adhered to the culture dish, were cultured/recovered with HydroCelI(TM)/Simple pipetting, with RepCelI(TM)/pipetting at 4 degrees C, or on normal plastic culture dishes with trypsinization or by scraping at 4 degrees C, respectively. The exocytosis of RBL-2H3 cells was largely impaired by scraping, but only slightly by the treatment with pipetting on HydroCell(TM), on RepCell(TM) or with trypsin. The membrane ruffling of the cells prepared by the last three treatments induced by antigens appeared the same. However, the change of AR with cells prepared by trypsin and those by scraping at 4 degrees C were lower than those by HydroCell(TM) or RepCell(TM), suggesting that trypsin may harm molecules involved in cellular reactions. Thus, the methods of cell fixation and removal with HydroCell(TM) or RepCell(TM) should enable us to analyze various reactions in either adherent or non-adherent cells by SPR.
言語 en
出版者
出版者 Elsevier Advanced Technology
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
出版タイプ
出版タイプ AO
出版タイプResource http://purl.org/coar/version/c_b1a7d7d4d402bcce
関連情報
識別子タイプ DOI
関連識別子 10.1016/j.bios.2007.07.005
関連情報
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 http://dx.doi.org/10.1016/j.bios.2007.07.005
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0956-5663
収録物識別子
収録物識別子タイプ NCID
収録物識別子 AA10739666
開始ページ
開始ページ 562
書誌情報 Biosensors & Bioelectronics
Biosensors & Bioelectronics

巻 23, 号 4, p. 562-567, 発行日 2007-11
旧ID 21470
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