| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Vanadium-binding proteins (Vanabins) from a vanadium-rich ascidian Ascidia sydneiensis samea |
|
言語 |
en |
| 作成者 |
Ueki, Tatsuya
Adachi, Takahiro
Kawano, Sonoko
Aoshima, Masato
Yamaguchi, Nobuo
Kanamori, Kan
Michibata, Hitoshi
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| アクセス権 |
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|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2003 Elsevier Science B.V. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Ascidian |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Vanadium |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Metal accumulation |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
460 |
| 内容記述 |
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|
内容記述 |
Since the beginning of the last century, it has been known that ascidians accumulate high levels of a transition metal, vanadium, in their blood cells, although the mechanism for this curious biological function remains unknown. Recently, we identified three vanadium-binding proteins (vanabins), previously denoted as vanadium-associated proteins (Kanda et al., 1997), from the cytoplasm fraction of vanadium-containing blood cells (vanadocytes) of the vanadium-rich ascidian Ascidia sydneiensis samea. Here, we describe the cloning, expression, and analysis of the metal-binding ability of vanabins. Recombinant proteins of two independent but related vanabins, vanabin1 and vanabin2, bound to 10 and 20 vanadium(IV) ions with dissociation constants of 2.1 × 10-5 M and 2.3 × 10-5 M, respectively. The binding of vanadium(IV) to these vanabins was inhibited by the addition of copper(II) ions, but not by magnesium(II) or molybdate(VI) ions. Vanabins are the first proteins reported to show specific binding to vanadium ions; this should provide a clue to resolving the problem regarding the selective accumulation of vanadium in ascidians. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier Science B.V. |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/S0167-4781(03)00036-8 |
| 関連情報 |
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|
識別子タイプ |
PMID |
|
|
関連識別子 |
12697328 |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/S0167-4781(03)00036-8 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0167-4781 |
| 開始ページ |
|
|
開始ページ |
43 |
| 書誌情報 |
Biochimica et biophysica acta
Biochimica et biophysica acta
巻 1626,
p. 43-50,
発行日 2003
|
| 旧ID |
24 |
BBA_1626_43-50_2003.pdf (273.0 KB)
