| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Effects of pressure on enzyme function of Escherichia coli dihydrofolate reductase |
|
言語 |
en |
| 作成者 |
Ohmae, Eiji
Tatsuta, Mineyuki
Abe, Fumiyoshi
Kato, Chiaki
Tanaka, Naoki
Kunugi, Shigeru
Gekko, Kunihiko
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2008 Elsevier B.V. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Dihydrofolate reductase |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Enzyme activity |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
High pressure |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Volume change |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
460 |
| 内容記述 |
|
|
内容記述 |
To elucidate the effects of pressure on the function of Escherichia coli dihydrofolate reductase (DHFR), the enzyme activity and the dissociation constants of substrates and cofactors were measured at pressures up to 250 MPa at 25ºC and pH 7.0. The enzyme activity decreased with increasing pressure, accompanying the activation volume of 7.8 ml mol-1. The values of the Michaelis constant (Km) for dihydrofolate and NADPH were slightly higher at 200 MPa than at atmospheric pressure. The hydride-transfer step was insensitive to pressure, as monitored by the effects of the deuterium isotope of NADPH on the reaction velocity. The dissociation constants of substrates and cofactors increased with pressure, producing volume reductions from 6.5 ml mol-1 (tetrahydrofolate) to 33.5 ml mol-1 (NADPH). However, the changes in Gibbs free energy with dissociation of many ligands showed different pressure dependences below and above 50 MPa, suggesting conformational changes of the enzyme at high pressure. The enzyme function at high pressure is discussed based on the volume levels of the intermediates and the candidates for the rate-limiting process. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier Science BV |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.bbapap.2008.04.005 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.bbapap.2008.04.005 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1570-9639 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11685085 |
| 開始ページ |
|
|
開始ページ |
1115 |
| 書誌情報 |
Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics
Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics
巻 1784,
号 7-8,
p. 1115-1121,
発行日 2008-07
|
| 旧ID |
25975 |
BBA-ProteinsProteomics_1784_1115.pdf (327.5 KB)
