| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
The conserved Wdr8-hMsd1/SSX2IP complex localises to the centrosome and ensures proper spindle length and orientation |
|
言語 |
en |
| 作成者 |
Hori, Akiko
Morand, Agathe
Ikebe, Chiho
Frith, David
Snijders, Ambrosius P.
Toda, Takashi
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
© 2015 The Francis Crick Insitute. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Bipolar spindle |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Centriolar satellites |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Centriole |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Centrosome |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Fission yeast |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Spindle pole body (SPB) |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Super resolution microscopy |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
WD repeats |
| 内容記述 |
|
|
内容記述 |
The centrosome plays a pivotal role in a wide range of cellular processes and its dysfunction is causally linked to many human diseases including cancer and developmental and neurological disorders. This organelle contains more than one hundred components, and yet many of them remain uncharacterised. Here we identified a novel centrosome protein Wdr8, based upon the structural conservation of the fission yeast counterpart. We showed that Wdr8 constitutively localises to the centrosome and super resolution microscopy uncovered that this protein is enriched at the proximal end of the mother centriole. Furthermore, we identified hMsd1/SSX2IP, a conserved spindle anchoring protein, as one of Wdr8 interactors by mass spectrometry. Wdr8 formed a complex and partially colocalised with hMsd1/SSX2IP. Intriguingly, knockdown of Wdr8 or hMsd1/SSX2IP displayed very similar mitotic defects, in which spindle microtubules became shortened and misoriented. Indeed, Wdr8 depletion resulted in the reduced recruitment of hMsd1/SSX2IP to the mitotic centrosome, though the converse is not true. Together, we propose that the conserved Wdr8-hMsd1/SSX2IP complex plays a critical role in controlling proper spindle length and orientation. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
T.T. and A.P.S were supported by Cancer Research UK. |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Supplementary data related to this article can be found at http://dx.doi.org/10.1016/j.bbrc.2015.10.169. |
| 出版者 |
|
|
出版者 |
Elsevier |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.bbrc.2015.10.169 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
26545777 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.bbrc.2015.10.169 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0006-291X |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1090-2104 |
| 開始ページ |
|
|
開始ページ |
39 |
| 書誌情報 |
Biochemical and Biophysical Research Communications
Biochemical and Biophysical Research Communications
巻 468,
号 1-2,
p. 39-45,
発行日 2015-12
|
| 旧ID |
48968 |
BiochemBiophysResCommun_468_39.pdf (1.2 MB)
