| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Structural determinants for the action of grayanotoxin in D1 S4.S5 and D4 S4.S5 intracellular linkers of sodium channel α-subunits |
|
言語 |
en |
| 作成者 |
Maejima, Hiroshi
Kinoshita, Eiji
Yuki, Tsunetsugu
Yakehiro, Masuhide
Seyama, Issei
Yamaoka, Kaoru
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2002 Elsevier Ltd. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Grayanotoxin |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Sodium channel |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
S4.S5 linker |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Whole-cell patch clamp |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
HEK 293 |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
460 |
| 内容記述 |
|
|
内容記述 |
We located a novel binding site for grayanotoxin on the cytoplasmic linkers of voltage-dependent cardiac (rH1) or skeletalmuscle (l1) Nat channel isoforms (segments S4.S5 in domains D1 and D4), using the alanine scanning substitution method. GTXmodification of Nat channels, transiently expressed in HEK 293 cells, was evaluated under whole-cell voltage clamp, from the ratio of maximum chord conductance for modified and unmodified Nat channels. In l1, mutations K237A, L243A, S246A, K248A, K249A, L250A, S251A, or T1463A, caused a moderate, but statistically significant decrease in this ratio. On making corresponding mutations in rH1, only L244A dramatically reduced the ratio. Because in l1, the serine at position 251 is the only heterologous residue with respect to rH1 (Ala-252), we made a double mutant L243A&S251A to match the sequence of l1 and rH1 in S4.S5 linkers of both domains. This double mutation resulted in a significant decrease in the ratio, to the same extent as L244A substitution in rH1 did, indicating that the site at Leu-244 in rH1 or at Leu-243 in l1 is a novel one, exhibiting a synergistic effect of grayanotoxin. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/S0006-291X(02)00702-7 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
12150970 |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/S0006-291X(02)00702-7 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0006-291X |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00564395 |
| 開始ページ |
|
|
開始ページ |
452 |
| 書誌情報 |
Biochemical and Biophysical Research Communications
Biochemical and Biophysical Research Communications
巻 295,
号 2,
p. 452-457,
発行日 2002
|
| 旧ID |
17039 |
BBRC_295_452.pdf (600.4 KB)
