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Identification of RNA regions that determine temperature sensitivities in betanodaviruses

https://hiroshima.repo.nii.ac.jp/records/2006315
https://hiroshima.repo.nii.ac.jp/records/2006315
6597f5a5-cb98-451b-b393-19a6abfcdc8f
名前 / ファイル ライセンス アクション
ArchVirol_155_1597.pdf ArchVirol_155_1597.pdf (1.8 MB)
Item type デフォルトアイテムタイプ_(フル)(1)
公開日 2023-03-18
タイトル
タイトル Identification of RNA regions that determine temperature sensitivities in betanodaviruses
言語 en
作成者 Hata, Naomi

× Hata, Naomi

en Hata, Naomi

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Okinaka, Yasushi

× Okinaka, Yasushi

en Okinaka, Yasushi

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Iwamoto, Tokinori

× Iwamoto, Tokinori

en Iwamoto, Tokinori

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Kawato, Yasuhiko

× Kawato, Yasuhiko

en Kawato, Yasuhiko

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Mori, Koh-Ichiro

× Mori, Koh-Ichiro

en Mori, Koh-Ichiro

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Nakai, Toshihiro

× Nakai, Toshihiro

en Nakai, Toshihiro

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
権利情報 Copyright (c) 2010 Springer
主題
主題Scheme Other
主題 betanodavirus
主題
主題Scheme Other
主題 temperature sensitivity
主題
主題Scheme Other
主題 reassortant
主題
主題Scheme Other
主題 chimeric virus
主題
主題Scheme Other
主題 viral growth
主題
主題Scheme Other
主題 RNA replication
主題
主題Scheme NDC
主題 460
内容記述
内容記述 Betanodaviruses, the causative agents of viral nervous necrosis in marine fish, have bipartite positive-sense RNA genomes. The larger genomic segment, RNA1 (similar to 3.1 kb), encodes an RNA-dependent RNA polymerase (protein A), and the smaller genomic segment RNA2 (similar to 1.4 kb) codes for the coat protein. These viruses can be classified into four genotypes, designated striped jack nervous necrosis virus (SJNNV), redspotted grouper nervous necrosis virus (RGNNV), tiger puffer nervous necrosis virus (TPNNV), and barfin flounder nervous necrosis virus (BFNNV), based on similarities in their partial RNA2 sequences. The optimal temperatures for the growth of these viruses are 20-25A degrees C (SJNNV), 25-30A degrees C (RGNNV), 20A degrees C (TPNNV), and 15-20A degrees C (BFNNV). However, little is known about the mechanisms underlying the temperature sensitivity of these viruses. We first constructed two reassortants between SJNNV and RGNNV to test their temperature sensitivity. The levels of viral growth and RNA replication of these reassortants and parental viruses in cultured fish cells were similar at 25A degrees C. However, the levels of all of the viruses but RGNNV were markedly reduced at 30A degrees C. These results indicate that both RNA1 and RNA2 control the temperature sensitivity of betanodaviruses by modulating RNA replication or earlier viral growth processes. We then constructed ten mutated RGNNVs, the RNA1 segments of which were chimeric between SJNNV and RGNNV, and showed that only chimeric viruses bearing the RGNNV RNA1 region, encoding amino acid residues 1-445, grew similarly to the parental RGNNV at 30A degrees C. This portion of protein A is known to serve as a mitochondrial-targeting signal rather than functioning as an enzymatic domain.
言語 en
出版者
出版者 Springer Wien
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
出版タイプ
出版タイプ AO
出版タイプResource http://purl.org/coar/version/c_b1a7d7d4d402bcce
関連情報
関連名称 The original publication is available at www.springerlink.com
関連情報
識別子タイプ DOI
関連識別子 10.1007/s00705-010-0736-7
関連情報
識別子タイプ DOI
関連識別子 http://dx.doi.org/10.1007/s00705-010-0736-7
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0304-8608
収録物識別子
収録物識別子タイプ NCID
収録物識別子 AA00548901
開始ページ
開始ページ 1597
書誌情報 Archives of Virology
Archives of Virology

巻 155, 号 10, p. 1597-1606, 発行日 2010-10
旧ID 30810
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