| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Expression and activity of Runx2 mediated by hyaluronan during chondrocyte differentiation |
|
言語 |
en |
| 作成者 |
Tanne, Yuki
Tanimoto, Kotaro
Tanaka, Nobuaki
Ueki, Masashi
Lin, Y. Y.
Ohkuma, S
Kamiya, Takashi
Tanaka, Eiji
Tanne, Kazuo
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2007 Elsevier Ltd |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Runt-related gene 2 (Runx2) |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Hyaluronan (HA) |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Chondrocyte |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Differentiation |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
HAoligo |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
|
|
内容記述 |
During endochondral ossification, the production of hyaluronan (HA) is strictly and selectively regulated by chondrocytes, with a temporal peak at the hypertrophic stage. This study was conducted to clarify the effects of HA on expression and activity of runt-related gene 2 (Runx2), a potent transcription factor for chondrocyte differentiation in hypertrophic chondrocytes. Immature chondrocytes from an ATDC5 cell line were cultured and differentiated in DMEM/Ham's F12 with pre-defined supplements. Using real-time PCR, the gene expressions of type II collagen, MMP-13, HAS2, and Runx2 in cultured chondrocytes were analysed from days 0 to 18 of cell differentiation. The activity and expression of Runx2 in hypertrophic chondrocytes were analysed after the treatment with HA oligosaccharide (HAoligo) using AML-3/Runx2 binding, real-time PCR and Western blot analysis. The effects of pre-incubation of anti-CD44 antibody on Runx2 expression were also examined. Expression of type X collagen and Runx2 mRNAs reached a maximum at the terminal differentiation of chondrocytes. The activity and expression of Runx2 was significantly inhibited in hypertrophic chondrocytes treated with HAoligo compared to the untreated controls. High molecular weight-HA did not affect the expression or activity of Runx2. The expression of Runx2 mRNA was significantly decreased in hypertrophic chondrocytes treated with anti-CD44 antibody. These results suggest that HAoligo may affect the terminal differentiation of chondrocytes during the endochondral ossification by inhibiting the expression and activity of Runx2. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier Ltd |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.archoralbio.2007.12.007 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
18242579 |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.archoralbio.2007.12.007 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0003-9969 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00548457 |
| 開始ページ |
|
|
開始ページ |
478 |
| 書誌情報 |
Archives of Oral Biology
Archives of Oral Biology
巻 53,
号 5,
p. 478-487,
発行日 2008-05
|
| 旧ID |
25579 |
ArcOralBiol_53_478.pdf (2.2 MB)
