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Ecophysiological interaction between nitrifying bacteria and heterotrophic bacteria in autotrophic nitrifying biofilms as determined by microautoradiography-fluorescence in situ hybridization

https://hiroshima.repo.nii.ac.jp/records/2006296
https://hiroshima.repo.nii.ac.jp/records/2006296
cf23821e-29c9-4195-abde-c80fb0305d36
名前 / ファイル ライセンス アクション
AEM_70_1641.pdf AEM_70_1641.pdf (2.7 MB)
Item type デフォルトアイテムタイプ_(フル)(1)
公開日 2023-03-18
タイトル
タイトル Ecophysiological interaction between nitrifying bacteria and heterotrophic bacteria in autotrophic nitrifying biofilms as determined by microautoradiography-fluorescence in situ hybridization
言語 en
作成者 Kindaichi, Tomonori

× Kindaichi, Tomonori

en Kindaichi, Tomonori

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Ito, Tsukasa

× Ito, Tsukasa

en Ito, Tsukasa

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Okabe, Satoshi

× Okabe, Satoshi

en Okabe, Satoshi

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
権利情報 Copyright (c) American Society for Microbiology
主題
主題Scheme NDC
主題 510
内容記述
内容記述 Ecophysiological interactions between the community members (i.e., nitrifiers and heterotrophic bacteria) in a carbon-limited autotrophic nitrifying biofilm fed only NH4+ as an energy source were investigated by using a full-cycle 16S RRNA approach followed by microautoradiography (MAR)-fluorescence in situ hybridization (FISH). Phylogenetic differentiation (identification) of heterotrophic bacteria was performed by 16S rRNA gene sequence analysis, and FISH probes were designed to determine the community structure and the spatial organization (i.e., niche differentiation) in the biofilm. FISH analysis showed that this autotrophic nitrifying biofilm was composed of 50% nitrifying bacteria (ammonia-oxidizing bacteria [AOB] and nitrite-oxidizing bacteria [NOB]) and 50% heterotrophic bacteria, and the distribution was as follows: members of the alpha subclass of the class Proteobacteria (α-Proteobacteria), 23%; γ-Proteobacteria, 13%; green nonsulfur bacteria (GNSB), 9%; Cytophaga-Flavobacterium-Bacteroides (CFB) division, 2%; and unidentified (organisms that could not be hybridized with any probe except EUB338), 3%. These results indicated that a pair of nitrifiers (AOB and NOB) supported a heterotrophic bacterium via production of soluble microbial products (SMP). MAR-FISH revealed that the heterotrophic bacterial community was composed of bacteria that were phylogenetically and metabolically diverse and to some extent metabolically redundant, which ensured the stability of the ecosystem as a biofilm. α- and γ -Proteobacteria dominated the utilization of [14C]acetic acid and 14C-amino acids in this biofilm. Despite their low abundance (ca. 2%) in the biofilm community, members of the CFB cluster accounted for the largest fraction (ca. 64%) of the bacterial community consuming N-acetyl-D-[1- 14C]glucosamine (NAG). The GNSB accounted for 9% of the 14C-amino acid-consuming bacteria and 27% of the [ 14C]NAG-consuming bacteria but did not utilize [14C] acetic acid. Bacteria classified in the unidentified group accoun
言語 en
出版者
出版者 American Society for Microbiology
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
関連情報
識別子タイプ DOI
関連識別子 10.1128/AEM.70.3.1641-1650.2004
関連情報
識別子タイプ DOI
関連識別子 http://dx.doi.org/10.1128/AEM.70.3.1641-1650.2004
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0099-2240
収録物識別子
収録物識別子タイプ NCID
収録物識別子 AA00543249
開始ページ
開始ページ 1641
書誌情報 Applied and Environmental Microbiology
Applied and Environmental Microbiology

巻 70, 号 3, p. 1641-1650, 発行日 2004-03
旧ID 20780
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