| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Reciprocating-flow ATP amplification system for increasing the number of amplification cycles |
|
言語 |
en |
| 作成者 |
Satoh, Tetsuya
Tsuruta, Kosuke
Shinoda, Yasuharu
Hirota, Ryuichi
Noda, Kenichi
Kuroda, Akio
Murakami, Yuji
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2009 Elsevier Inc. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
ATP amplification |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Reciprocating-flow system |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Bioluminescence assay |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
460 |
| 内容記述 |
|
|
内容記述 |
We constructed a novel ATP amplification reactor using a reciprocating-flow system to increase the number of ATP amplification cycles without an increase in backpressure. We previously reported a continuous-flow ATIP amplification system that effectively and quantitatively amplified ATP and increased the sensitivity of a quantitative bioluminescence assay. However, it was difficult to increase the number of amplification cycles due to backpressure in the system. Because addition of immobilized adenylate kinase (ADK) and pyruvate kinase (PK) columns increased backpressure, the maximum number of ATP amplification cycles within Column durability was only 4. In this Study, ATP amplification was performed using a reciprocating-flow system, and 10 cycles of ATP amplification could be achieved without an increase in backpressure. As a result, ATP was amplified more than 100-fold after 10 cycles of reciprocating flow. The gradient of ATP amplification was approximately 1.76(N). The backpressure oil the columns was 0.03 MPa in 1-10 ATIP amplification cycles, and no increases in backpressure were observed. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Academic Press INC |
| 出版者 |
|
|
出版者 |
Elsevier Science |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.ab.2009.08.025 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.ab.2009.08.025 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0003-2697 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00524867 |
| 開始ページ |
|
|
開始ページ |
161 |
| 書誌情報 |
Analytical Biochemistry
Analytical Biochemistry
巻 395,
号 2,
p. 161-165,
発行日 2009-12-15
|
| 旧ID |
28856 |
AnalBiochem_395_161.pdf (1.3 MB)
