| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Detection of the Gua/Cyt-to-Cyt/Gua mutation in a Gua/Cyt-lined sequence using Zn2+–cyclen polyacrylamide gel electrophoresis |
|
言語 |
en |
| 作成者 |
Kinoshita, Eiji
Kinoshita-Kikuta, Emiko
Yoshimoto, Miki
Koike, Tohru
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2008 Elsevier Inc. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Guanine |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Cytosine |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
SNP |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Mutation |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Heteroduplex |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Zn2+–cyclen |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Electrophoresis |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 内容記述 |
|
|
内容記述 |
We have previously reported a method for the detection of single-nucleotide polymorphisms by polyacrylamide gel electrophoresis (PAGE) with an additive Zn2+–cyclen complex (cyclen = 1,4,7,10-tetraazacyclododecan), called Zn2+–cyclen–PAGE. The method is based on the difference in mobility of mutant DNA (in the same length) in PAGE, which is due to Zn2+–cyclen binding to thymine bases accompanying a total charge decrease and a local conformation change of target DNA. In combination with a heteroduplexing technique, the method is more accurate, as shown by clear gel-shifting bands. However, the question remains of whether the Gua/Cyt-to-Cyt/Gua mutation, which is far apart from the Thy/Ade base (i.e., in a Gua/Cyt-lined sequence), can be detected by this Thy-dependent method. In this study, we determined the potency of Zn2+–cyclen–PAGE for the detection of the Gua/Cyt-to-Cyt/Gua single substitutions in some artificial Gua/Cyt-lined sequences derived from a human cardiac sodium channel gene, SCN5A. All Gua/Cyt-to-Cyt/Gua substitutions in the 28-set samples tested, which are 1 to 10 bases away from the nearest Thy/Ade, were successfully detected by designing DNA fragments of the appropriate length. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Academic Press Inc. |
| 出版者 |
|
|
出版者 |
Elsevier Science |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.ab.2008.05.040 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.ab.2008.05.040 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0003-2697 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00524867 |
| 開始ページ |
|
|
開始ページ |
122 |
| 書誌情報 |
Analytical Biochemistry
Analytical Biochemistry
巻 380,
号 1,
p. 122-127,
発行日 2008-09-01
|
| 旧ID |
25927 |
AnalBiochem_380_122.pdf (3.6 MB)
