| Item type |
デフォルトアイテムタイプ_（フル）(1) |
| 公開日 |
2023-03-18 |
| タイトル |
|
|
タイトル |
Simultaneous monitoring of inorganic cations, amines and amino acids in human sweat by capillary electrophoresis |
|
言語 |
en |
| 作成者 |
Hirokawa, Takeshi
Okamoto, Hikaru
Gosyo, Yoshinori
Tsuda, Takao
Timerbaev, Andrei R.
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
権利情報 |
Copyright (c) 2006 Elsevier B.V. |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Human sweat |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Capillary electrophoresis |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Sampling |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Cations |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Amino acids |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
Amines |
| 主題 |
|
|
主題Scheme |
NDC |
|
主題 |
430 |
| 内容記述 |
|
|
内容記述 |
The determination of cationic constituents of sweat is widely recognized as a difficult analytical task due to its complex composition and minute sample volumes available for the individual analysis. Capillary electrophoresis (CE) has been evaluated as a simple routine method to measure sweat metal cations, biogenic amines, and amino acids using a sampling procedure previously developed in one of collaborative teams. The carrier electrolyte, which consisted of 10 mM 4-methylbenzylamine, 6.5 mM α-hydroxyisobutyric acid, and 2 mM 18-crown-6 at pH 4.25 allowed the separation of five cations (NH4+, K+, Ca2+, Na+, Mg2+) and four amino acids (ornithine, histidine, lysine, arginine) to be completed in about 13 min with a positive polarity of the applied voltage (30 kV). By increasing the sample volume (due to employing hydrodynamic instead of hydrostatic injection mode), it was also possible to detect indirect UV signals of Zn2+, diethanolamine, and trietanolamine. Sweat samples were collected from the fingers and forearms of three healthy male volunteers and analyzed by CE. A good repeatability and reproducibility of peak area responses based on five intraday and three inter-day assays (average %RSD less than 3.5 and 2.5, respectively) were obtained. The limits of detection were in the range of 3.2-5.8 μM for alkali and alkaline-earth cations (hydrostatic injection) and 0.27-0.79 μM for other target analytes (hydrodynamic injection). The analytical results for particular analytes were found to vary, depending on the sampling spot and individual, but in general correspond well to clinical concentration ranges. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Elsevier Science B.V. |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AO |
|
出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.aca.2006.07.077 |
| 関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.aca.2006.07.077 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0003-2670 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00524834 |
| 開始ページ |
|
|
開始ページ |
83 |
| 書誌情報 |
Analytica Chimica Acta
Analytica Chimica Acta
巻 581,
号 1,
p. 83-88,
発行日 2007-01-02
|
| 旧ID |
20606 |
ACA_581-1_83.pdf (200.0 KB)
